Cytomegalovirus Transmission in Pregnant Women

孕妇巨细胞病毒传播

• 批准号: 7432597
• 负责人: BETH C MARSHALL
• 金额: $ 13.31万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7432597
• 关键词: Advisory Committees; Behavior Therapy; Birth; Child; Clinical; Clinical Research; Conceptions; Cytomegalovirus; Cytomegalovirus Infections; Development; Disease; Exposure to; Fetus; Genome; Genomics; Goals; Hearing Impaired Persons; Immunologic Factors; Immunologics; Infant; Infection; Institution; Instruction; Intervention; Knowledge; Marshal; Mentally Disabled Persons; Mentors; Methods; Mothers; Newborn Infant; Pattern; Personal Satisfaction; Pregnancy; Pregnant Women; Program Development; Purpose; Rate; Research; Research Personnel; Research Project Grants; Research Training; Serological; Siblings; Source; Syndrome; Testing; Training; Vaccines; Virus; Virus Shedding; Woman; career; congenital cytomegalovirus; congenital infection; experience; member; prevent; response; transmission process; vaccine development

DESCRIPTION (provided by applicant): The purpose of this proposal is to provide a strong mentoring experience and didactic training to afford Dr. Beth Marshall the opportunity to attain her long term goal of becoming an independent investigator in an academic institution. To this end, we propose a multifaceted career development program consisting of: 1) didactic instruction via coursework and seminar attendance; 2) extensive mentoring in the form of 2 overseeing mentors and a 6-member Career Advisory Committee; 3) research training via a substantial and well-rounded clinical research project. The research we propose will test the hypothesis that pregnant women who have been infected with CMV one or more years prior to pregnancy (seropositive) are not completely protected against delivering congenially infected infants because they become reinfected with CMV just before or during pregnancy. Infection of the fetus with cytomegalovirus (CMV) results in approximately 8,000 infants born each year who will be mentally retarded and/or deaf. These effects are mainly due to primary maternal CMV infection during pregnancy. Although less common, seropositive women can also give birth to congenially infected infants, but what is not known is whether these women have sustained a reactivation of CMV or reinfection with another strain. The answer to this question has significant implications for both vaccine development as well as intervention strategies. To determine whether reinfection is the primary source of congenital infection in seropositive women, we will compare congenital infection rates in 3 groups of seropositive pregnant women: women with children shedding CMV, women without children shedding CMV, and women who have seroconverted just prior to pregnancy. Reinfection will be defined by demonstrating identical genomic patterns of virus shed by an older sibling (infected during the course of the study) and the newborn. Additionally, we will establish the immunologic responses and virologic aspects of those seropositive women who give birth to infected newborns, as this information is important for understanding the factors involved in the transmission of virus to the fetus, and ultimately for the development of an efficacious vaccine to prevent congenital CMV.

描述（由申请人提供）：本提案的目的是提供强有力的指导经验和教学培训，使贝丝·马歇尔博士有机会实现成为学术机构独立调查员的长期目标。为此，我们提出了一个多方面的职业发展计划，包括：1）通过课程作业和参加研讨会进行教学指导； 2) 以 2 名监督导师和 6 名职业咨询委员会的形式提供广泛的指导； 3) 通过实质性且全面的临床研究项目进行研究培训。我们提出的研究将检验以下假设：在怀孕前一年或一年以上感染 CMV（血清反应阳性）的孕妇并不能完全避免生下先天感染的婴儿，因为她们在怀孕前或怀孕期间再次感染 CMV。胎儿感染巨细胞病毒 (CMV) 会导致每年大约 8,000 名出生的婴儿出现智力障碍和/或耳聋。这些影响主要是由于妊娠期间原发性母体巨细胞病毒感染所致。虽然不太常见，但血清反应呈阳性的女性也可能生下先天感染的婴儿，但尚不清楚这些女性是否经历了巨细胞病毒的重新激活或另一种病毒株的再次感染。这个问题的答案对疫苗开发和干预策略都具有重大影响。为了确定再感染是否是血清阳性女性先天性感染的主要来源，我们将比较 3 组血清阳性孕妇的先天性感染率：有子女排出 CMV 的妇女、没有子女排出 CMV 的妇女以及怀孕前发生血清转化的妇女。 再感染将通过证明年长的兄弟姐妹（在研究过程中感染）和新生儿排出的病毒的相同基因组模式来定义。此外，我们将确定那些生下受感染新生儿的血清反应呈阳性的妇女的免疫反应和病毒学方面，因为这些信息对于了解病毒传播给胎儿的因素非常重要，并最终有助于开发有效的疫苗来预防先天性巨细胞病毒。