Temperature Entrainment of Drosophila Circadian Rhythms

本提案的长期目标是了解环境温度波动 同步果蝇的生物钟虽然无脊椎动物和哺乳动物的许多成分 分子生物钟已经确定，相当少的是知道如何从信息 环境破坏了内部分子起搏器。在这个项目中，温度的影响 果蝇昼夜节律行为的波动将被表征。果蝇遗传学的力量 用于识别昼夜节律同步所需的神经元结构和昼夜节律钟基因 温度循环。最后，生物化学技术将用于阐明分子机制 起搏器通过其响应温度输入。确定温度和其他 环境输入被整合在一个简单的模型系统中，将提供关于外部输入如何 可以独立和协同地影响复杂行为（如睡眠）的时间。这 最终将导致更好地理解和治疗与昼夜节律有关的疾病， 如睡眠障碍，轮班工作相关的并发症，季节性情感和双相情感障碍。

The long term objective of this proposal is to understand how environmental temperature fluctuations synchronize the Drosophila circadian clock. While many of the components of invertebrate and mammalian molecular circadian clocks have been identified, considerably less is known about how information from the environment synchronizes the internal molecular pacemaker. In this project, the effect of temperature fluctuations on Drosophila circadian behavior will be characterized. The power of Drosophila genetics will be used to identify neuronal structures and circadian clock genes required for circadian rhythm synchronization by temperature cycles. Finally, biochemical techniques will be used to elucidate the molecular mechanisms by which the pacemaker responds to temperature inputs. Determining how temperature and other environmental inputs are integrated in a simple model system will provide information on how external inputs can act independently and synergistically to affect the timing of complex behaviors such as sleep. This should ultimately lead to a better understanding and treatment of illnesses linked to circadian rhythms, such as sleep disorders, shift-work associated complications, seasonal affective and bipolar disorder.