Temperature Entrainment of Drosophila Circadian Rhythms

果蝇昼夜节律的温度夹带

• 批准号: 7339835
• 负责人: ANIA C BUSZA
• 金额: $ 2.88万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-26 至 2009-08-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339835
• 关键词: Affect; Behavior; Biochemical; Biological Clocks; Biological Models; Bipolar Disorder; Circadian Rhythms; Clinical Pathology; Clock protein; Complex; Condition; Cues; Disadvantaged; Drosophila genus; Drosophila melanogaster; Environment; Feedback; Genes; Genetic; Heating; Hour; Human; Invertebrates; Lead; Light; Link; Molecular; Molecular Genetics; Monitor; Motor Activity; Neurons; Organism; Pacemakers; Peripheral; Personal Satisfaction; Phase; Physiologic pulse; Physiology; Pulse taking; Reporting; Sense Organs; Shift-Work Sleep Disorder; Sleep; Structure; Techniques; Temperature; Testing; Time; circadian pacemaker; day; fly; light effects

The long term objective of this proposal is to understand how environmental temperature fluctuations synchronize the Drosophila circadian clock. While many of the components of invertebrate and mammalian molecular circadian clocks have been identified, considerably less is known about how information from the environment synchronizes the internal molecular pacemaker. In this project, the effect of temperature fluctuations on Drosophila circadian behavior will be characterized. The power of Drosophila genetics will be used to identify neuronal structures and circadian clock genes required for circadian rhythm synchronization by temperature cycles. Finally, biochemical techniques will be used to elucidate the molecular mechanisms by which the pacemaker responds to temperature inputs. Determining how temperature and other environmental inputs are integrated in a simple model system will provide information on how external inputs can act independently and synergistically to affect the timing of complex behaviors such as sleep. This should ultimately lead to a better understanding and treatment of illnesses linked to circadian rhythms, such as sleep disorders, shift-work associated complications, seasonal affective and bipolar disorder.

该提案的长期目标是了解环境温度波动如何 同步果蝇生物钟。虽然无脊椎动物和哺乳动物的许多成分 分子昼夜节律钟已经被识别出来，但人们对如何从分子生物钟中获得信息知之甚少。 环境同步内部分子起搏器。在本项目中，温度的影响 果蝇昼夜节律行为的波动将被表征。果蝇遗传学的力量将是 用于识别昼夜节律同步所需的神经元结构和生物钟基因 通过温度循环。最后，生化技术将用于阐明分子机制 起搏器通过它响应温度输入。确定温度和其他 环境输入集成在一个简单的模型系统中，将提供有关外部输入如何进行的信息 可以独立和协同作用来影响睡眠等复杂行为的时间。这 最终应该会导致更好地理解和治疗与昼夜节律相关的疾病，例如 如睡眠障碍、轮班工作相关并发症、季节性情感障碍和双相情感障碍。