Core--Biochemistry

核心--生物化学

• 批准号: 7433781
• 负责人: Jennifer S Pollock
• 金额: $ 29.59万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7433781
• 关键词: Angiotensins; Biochemical; Biochemistry; Biological Assay; Blood Pressure; Blood Vessels; Collaborations; Collagen Type IV; Collection; Core Facility; Corticosterone; Creatinine; Cyclic GMP; Development; Dinoprost; Dinoprostone; Electrolytes; Endothelin Receptor; Endothelin-1; Enzyme-Linked Immunosorbent Assay; Equipment; Evaluation; Fibronectins; Functional disorder; Hematoxylin and Eosin Staining Method; Histology; Human Resources; Hypertension; Impairment; Individual; Injury; Interleukin-6; Ion-Selective Electrodes; Kidney; Laboratories; Measurement; Mediation; Mesentery; Methods; Mus; Norepinephrine; Numbers; Perfusion; Periodic acid Schiff stain method; Plasma; Plasma Proteins; Preparation; Price; Principal Investigator; Procedures; Process; Prostaglandin-Endoperoxide Synthase; Proteins; Psychosocial Stress; Quality Control; Radioimmunoassay; Rattus; Records; Renal Tissue; Renin; Reporting; Research Personnel; Resource Development; Rho-associated kinase; Role; Sampling; Services; Signal Transduction; Staining method; Stains; Techniques; Tissue Extracts; Tissue Fixation; Tissue Sample; Tissue Stains; Tissues; Title; Training; Transforming Growth Factor beta; Trichrome stain method; Urine; Validation; Validity and Reliability; Work; analytical method; cost; cyclooxygenase 1; experience; intracellular protein transport; isoprostaglandin F2alpha type-III; programs; prostaglandin F1; protein localization location; response; salt sensitive; tissue fixing

The Biochemistry Core laboratory will provide biochemical assay services as well as histology and immunohistochemical analyses for the individual projects within the Program Project. Biochemical assays that will be performed include IL-6, IL-1beta, TNFalpha, TGFbeta, ET-1, microalbumin, norepinephrine, tissue total protein, plasma fibrinolytic activity, plasma renin, corticosterone, fibronectin, electrolytes, creatinine, 8- isoprostane, cGMP, 6-keto PGF(1alpha), PGE2, PGF(2alpha), TxB2 measurements in urine, plasma, and/or tissue extractsfrom mice and rats. Techniques will include radioimmunoassay, radioenzymatic assay, ELISA, and ion-selective electrodes. Histology and immunohistochemical analyses will provide hematoxylin & eosin (H&E), periodic acid-Schiff (PAS), Sirius red, blue trichrome, and specific immunohistochemical staining analyses for four of the projects in the Program Project. The core will also provide resources for development of additional assays and/or histological and immunohistological analyses as needed by individual investigators. The core will assure assay and histology quality control through validation procedures.

生物化学核心实验室将为计划项目中的各个项目提供生化分析服务以及组织学和免疫组织化学分析。将进行的生化测定包括小鼠和大鼠尿液、血浆和/或组织提取物中的IL-6、IL-1 β、TNF α、TGF β、ET-1、微量白蛋白、去甲肾上腺素、组织总蛋白、血浆纤溶活性、血浆肾素、皮质酮、纤连蛋白、电解质、肌酐、8-异前列烷、cGMP、6-酮PGF（1 α）、PGE 2、PGF（2 α）、TxB 2测量。技术将包括放射免疫测定、放射酶测定、ELISA和离子选择性免疫测定。 个电极组织学和免疫组织化学分析将提供苏木精和伊红（H&E）， 高碘酸-希夫（PAS），天狼星红，蓝三色，和特定的免疫组织化学染色分析的四个项目中的计划项目。该核心还将提供资源，用于开发个体研究者所需的其他检测和/或组织学和免疫组织学分析。核心将通过验证程序确保含量测定和组织学质量控制。