Core--Biochemistry

核心--生物化学

• 批准号: 7228249
• 负责人: Jennifer S Pollock
• 金额: $ 19.45万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7228249
• 关键词: angiotensin /renin /aldosterone hypertension; bioassay; biomedical facility; blood chemistry; blood flow measurement; electrodes; enzyme linked immunosorbent assay; histology; immunocytochemistry; laboratory mouse; laboratory rat; method development; radioimmunoassay; urinalysis

The Biochemistry Core laboratory will provide biochemical assay services as well as histology and immunohistochemical analyses for the individual projects within the Program Project. Biochemical assays that will be performed include IL-6, IL-1beta, TNFalpha, TGFbeta, ET-1, microalbumin, norepinephrine, tissue total protein, plasma fibrinolytic activity, plasma renin, corticosterone, fibronectin, electrolytes, creatinine, 8- isoprostane, cGMP, 6-keto PGF(1alpha), PGE2, PGF(2alpha), TxB2 measurements in urine, plasma, and/or tissue extractsfrom mice and rats. Techniques will include radioimmunoassay, radioenzymatic assay, ELISA, and ion-selective electrodes. Histology and immunohistochemical analyses will provide hematoxylin & eosin (H&E), periodic acid-Schiff (PAS), Sirius red, blue trichrome, and specific immunohistochemical staining analyses for four of the projects in the Program Project. The core will also provide resources for development of additional assays and/or histological and immunohistological analyses as needed by individual investigators. The core will assure assay and histology quality control through validation procedures.

生物化学核心实验室将为计划项目中的个别项目提供生化分析服务以及组织学和免疫组织化学分析。将进行的生化检测包括IL-6、IL-1β、TNFα、TGFβ、ET-1、微量白蛋白、去甲肾上腺素、组织总蛋白、血浆纤溶活性、血浆肾素、皮质酮、纤维连接蛋白、电解质、肌酐、8-异前列腺素、cGMP、6-keto PGF(1α)、PGE2、PGF(2α)、尿液、血浆和/或大鼠组织提取液中的TxB2。技术将包括放射免疫分析、放射性酶分析、酶联免疫吸附试验和离子选择。 电极。组织学和免疫组织化学分析将提供苏木精-伊红(H&E)， 对计划项目中的四个项目进行了高碘酸-席夫(PAS)、天狼星红、蓝三色和特异性免疫组织化学染色分析。该核心还将根据个别调查人员的需要，为开发其他化验和/或组织和免疫组织学分析提供资源。核心将通过验证程序确保化验和组织学质量控制。