New proteome technologies: mapping adult D. melanogaster

新的蛋白质组技术：绘制成年黑腹果蝇图谱

• 批准号: 7415048
• 负责人: DAVID E. CLEMMER
• 金额: $ 24.48万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7415048
• 关键词: Address; Adipose tissue; Adult; Affect; Age; Aging; Aging-Related Process; Amino Acids; Animal Model; Animals; Biological; Biological Models; Biology; Brain; Buffers; Characteristics; Charge; Chemicals; Complex; Complex Mixtures; Condition; Coupled; Data; Detection; Development; Digestion; Dimensions; Drosophila genus; Drosophila melanogaster; Elements; Event; Exhibits; Gases; Genetic; Genetic Models; Genome; Genomics; Genotype; Gonadal structure; Human; Huntington Disease; Informatics; Ions; Knock-out; Maps; Mass Spectrum Analysis; Measurement; Measures; Mind; Modeling; Molecular; Muscle; Mutation; Nerve Degeneration; Neurodegenerative Disorders; Neurologic; Noise; Numbers; Organism; Parkinson Disease; Peptides; Personal Satisfaction; Phase; Positioning Attribute; Process; Proteins; Proteome; Proteomics; Range; Series; Shapes; Staging; Structure; System; Techniques; Technology; Testing; Time; Transgenic Organisms; Tube; Variant; Work; analog; base; data management; design; fly; improved; instrument; interest; ion mobility; mass spectrometer; mutant; new technology; next generation; programs; protein aminoacid sequence; prototype; reproductive; research study; two-dimensional

DESCRIPTION (provided by applicant): This proposal describes a program for developing multidimensional analytical techniques for the analysis of complex biological mixtures that are extracted from Drosophila melanogaster as a function of the age of the organism. Preliminary data show that a new gas-phase ion mobility/time-of-flight mass spectrometry technique can be coupled to other separation techniques in order to separate and characterize complex mixtures of tryptic peptides obtained from digestion of soluble proteins. The gas-phase separation reduces spectral congestion and chemical noise associated with mass spectrometry alone, provides additional information about charge state and amino acid composition, and allows a parallel approach for obtaining fragmentation data (an important factor for assigning peptide sequence and identifying proteins). This proposal describes the further advancement of these technologies and their applications to the emerging field of proteomics. An aggressive plan that aims to map the proteome for the fundamental model Drosophila organism is proposed. This existence of a detailed proteomic map for this system is an important scientific step that will allow a range of genetic models to be investigated in detail. These models are chosen to display characteristics of diseased states (e.g., neurodegenerative conditions) for which genetic studies in humans are intractable.

描述（由申请人提供）：本提案描述了一项开发多维分析技术的计划，用于分析从黑腹果蝇中提取的复杂生物混合物，作为生物体年龄的函数。初步数据表明，一种新的气相离子迁移率/飞行时间质谱技术可以耦合到其他分离技术，以分离和表征复杂的混合物的胰蛋白酶肽从消化可溶性蛋白质。气相分离减少了与质谱法单独相关的光谱拥塞和化学噪声，提供了关于电荷状态和氨基酸组成的额外信息，并允许用于获得片段化数据（分配肽序列和鉴定蛋白质的重要因素）的并行方法。该提案描述了这些技术的进一步发展及其在新兴的蛋白质组学领域的应用。提出了一个积极的计划，旨在映射的基本模式果蝇生物体的蛋白质组。该系统的详细蛋白质组图谱的存在是一个重要的科学步骤，将允许详细研究一系列遗传模型。选择这些模型以显示患病状态的特征（例如，神经退行性疾病），对于这些疾病，人类的遗传学研究是难以解决的。

项目成果

Lifetime proteomic profiling of an A30P alpha-synuclein Drosophila model of Parkinson's disease.

帕金森病 A30P α-突触核蛋白果蝇模型的终生蛋白质组学分析。

• DOI: 10.1021/pr0700504
• 发表时间: 2007
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Xun,Zhiyin;Sowell,RenaA;Kaufman,ThomasC;Clemmer,DavidE
• 通讯作者: Clemmer,DavidE

Stable isotope labeling and label-free proteomics of Drosophila parkin null mutants.

• DOI: 10.1021/pr9006238
• 发表时间: 2009-10
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Xun, Zhiyin;Kaufman, Thomas C.;Clemmer, David E.
• 通讯作者: Clemmer, David E.

Insights into aging through measurements of the Drosophila proteome as a function of temperature.

通过测量果蝇蛋白质组作为温度的函数来了解衰老。

• DOI: 10.1016/j.mad.2010.08.004
• 发表时间: 2010
• 期刊: Mechanisms of ageing and development
• 影响因子: 5.3
• 作者: Robinson,RenaAS;Kellie,JohnF;Kaufman,ThomasC;Clemmer,DavidE
• 通讯作者: Clemmer,DavidE

Overtone mobility spectrometry: part 1. Experimental observations.

• DOI: 10.1016/j.jasms.2008.11.022
• 发表时间: 2009-05
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Kurulugama RT;Nachtigall FM;Lee S;Valentine SJ;Clemmer DE
• 通讯作者: Clemmer DE

Determination of cross sections by overtone mobility spectrometry: evidence for loss of unstable structures at higher overtones.

• DOI: 10.1021/jp1060123
• 发表时间: 2010-09-30
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY B
• 影响因子: 3.3
• 作者: Lee, Sunyoung;Ewing, Michael A.;Nachtigall, Fabiane M.;Kurulugama, Ruwan T.;Valentine, Stephen J.;Clemmer, David E.
• 通讯作者: Clemmer, David E.