Cytometry Core

细胞计数核心

• 批准号: 7480730
• 负责人: Beth Deirdre Jamieson-Karavodin
• 金额: $ 9.61万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7480730
• 关键词: Acquired Immunodeficiency Syndrome; Arts; Biological; Biological Assay; Budgets; Calibration; Cell Separation; Class; Color; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; Daily; Development; Educational process of instructing; Educational workshop; Ensure; Enzymes; Equipment; Expenditure; Flow Cytometry; Fostering; Funding; Guidelines; HIV; Health; Housing; Human Resources; Image Cytometry; Immunologist; Individual; Institutes; Lasers; Link; Magnetism; Maintenance; Measurement; Methods; Operative Surgical Procedures; Patients; Procedures; Research; Research Personnel; Resources; Role; Safety; Services; Techniques; Time; Training; Work; base; computer data analysis; concept; cost; cytokine; digital; enzyme linked immunospot assay; experience; improved; instrumentation; member; multidisciplinary; novel; posters; repaired

The UCLA Cytometry Core Facility (CCF), a campus-wide resource, provides state-of-the-art instrumentation and expertise at an affordable cost to UCLA researchers needing flow and ELISpot image cytometry. In 1988 the Jonsson Comprehensive Cancer Center established the resource, and since 1991 the Center For AIDS Research (CFAR) has provided additional support. Since 2000 Dr. Jamieson, an accomplished cellular immunologist, directs the CCF and is available to users for consultation. Ms. Schmid, the manager of the facility since 1988, is responsible for the CCF's daily operation and assists Dr. Jamieson with long-term planning. Ms. Schmid is an expert flow cytometrist, well known for her development of flow cytometry methods and her pivotal role in establishing biosafety guidelines for cell sorting. Since 2001 the CCF provides ELISpot assay services under the guidance of Dr. Uittenbogaart, a noted cellular immunologist with extensive expertise in cytokine measurements. The CCF has a single-laser FACScan, a dual-laser FACSCalibur, a three-laser BD LSR I, a modified, dual-laser, five-color FACScan, three, three-laser sorters, an autoMACS magnetic separator, and an off-line data analysis computer. An ImmunoSpot Analyzer, three, free-standing analyzers, and five biological safety cabinets are also available. Cytometric instrumentation is expensive and beyond the budget of most individual researchers. The CCF is easily accessible and is responsible for procurement, daily calibration, maintenance and repair of all instrumentation. CCF personnel have a combined 82 years of flow cytometry and 46 years of cytokine measurement experience. Expertise is available to investigators through one-on-one consultation, classes, a website, poster presentations, and open houses. Ms. Schmid and Dr. Uittenbogaart also work with researchers to adapt or develop assays to enable investigators to pursue research questions in novel ways. CCF services help researchers with varying amounts of experience in cytometry to perform high quality assays with minimal time expenditure. Workshops, seminars, and utilization of new assays ensure that researchers remain on the cutting edge of cytometry without investing their own funds or staff time on keeping current in the latest advances. During the last funding period a total of 250 investigators used the CCF; 40 of those were CFAR/AIDS Institute members representing 38% of use. This documents the role of the CCF in enhancing HIV research at UCLA with its potential contribution to improving the health of HIV-infected patients.

加州大学洛杉矶分校的细胞学核心设施(CCF)是一个校园范围的资源，提供最先进的仪器 加州大学洛杉矶分校的研究人员需要Flow和ELISpot图像细胞仪，而且成本合理。在……里面 1988年，琼森综合癌症中心建立了该资源，自1991年以来，该中心 艾滋病研究中心(CFAR)提供了额外的支持。自2000年以来，杰米森博士是一位成就卓著的细胞学专家 免疫学家，指导CCF，并可供用户咨询。施密德女士，公司的经理 自1988年以来，负责CCF的日常运作，并协助Jamieson博士长期 计划。施密德女士是一位专业的流式细胞仪专家，以她在流式细胞术方面的发展而闻名。 方法和她在建立细胞分选生物安全指南中的关键作用。自2001年以来，中华全国总工会 在Uittenbogaart博士的指导下提供ELISpot检测服务，Uittenbogaart博士是著名的细胞免疫学家， 在细胞因子测量方面拥有丰富的专业知识。CCF有一个单激光器FACScan，一个双激光器 FACSCalibur，三激光BD LSR I，改进型双激光，五色FACScan，三，三激光分选机， AutoMACS磁选机和离线数据分析计算机。一个免疫斑点分析仪，三， 还提供独立式分析仪和五个生物安全柜。细胞学仪器是 费用昂贵，超出了大多数研究人员的个人预算。CCF很容易到达，并且是 负责所有仪表的采购、日常校准、维护和维修。CCF人员 拥有82年的流式细胞仪和46年的细胞因子检测经验。专门知识 调查人员可以通过一对一咨询、课程、网站、海报演示和 开放参观。施密德女士和乌滕博加特博士还与研究人员合作，调整或开发检测方法 使研究人员能够以新颖的方式提出研究问题。CCF服务帮助研究人员 在细胞学方面有不同程度的经验，能够以最少的时间进行高质量的分析。 研讨会、研讨会和新分析方法的使用确保研究人员始终处于前沿 无需投入自己的资金或工作人员的时间来保持最新的进展。在.期间 在上一个资助期，共有250名调查人员使用了CCF；其中40人是CFAR/AIDS研究所 会员占使用量的38%。这份文件记录了CCF在加强加州大学洛杉矶分校艾滋病毒研究方面的作用 它对改善艾滋病毒感染者的健康具有潜在的贡献。