Cytometry Core

细胞计数核心

• 批准号: 8377981
• 负责人: Beth Deirdre Jamieson-Karavodin
• 金额: $ 16.31万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2013-03-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8377981
• 关键词: Acquired Immunodeficiency Syndrome; Biological; Biological Assay; Budgets; Calibration; Cell Separation; Color; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; Development; Educational process of instructing; Educational workshop; Ensure; Enzymes; Equipment; Expenditure; Flow Cytometry; Fostering; Funding; Guidelines; HIV; Health; Housing; Human Resources; Image Cytometry; Immunologist; Individual; Institutes; Lasers; Link; Magnetism; Maintenance; Measurement; Methods; Patients; Procedures; Research; Research Personnel; Resources; Role; Safety; Services; Techniques; Time; Training; Work; base; computer data analysis; cost; cytokine; digital; enzyme linked immunospot assay; experience; improved; instrumentation; member; multidisciplinary; novel; operation; posters; repaired; web site

The UCLA Cytometry Core Facility (CCF), a campus-wide resource, provides state-of-the-art instrumentation and expertise at an affordable cost to UCLA researchers needing flow and ELISpot image cytometry. In 1988 the Jonsson Comprehensive Cancer Center established the resource, and since 1991 the Center For AIDS Research (CFAR) has provided additional support. Since 2000 Dr. Jamieson, an accomplished cellular immunologist, directs the CCF and is available to users for consultation. Ms. Schmid, the manager of the facility since 1988, is responsible for the CCF's daily operation and assists Dr. Jamieson with long-term planning. Ms. Schmid is an expert flow cytometrist, well known for her development of flow cytometry methods and her pivotal role in establishing biosafety guidelines for cell sorting. Since 2001 the CCF provides ELISpot assay services under the guidance of Dr. Uittenbogaart, a noted cellular immunologist with extensive expertise in cytokine measurements. The CCF has a single-laser FACScan, a dual-laser FACSCalibur, a three-laser BD LSR I, a modified, dual-laser, five-color FACScan, three, three-laser sorters, an autoMACS magnetic separator, and an off-line data analysis computer. An ImmunoSpot Analyzer, three, free-standing analyzers, and five biological safety cabinets are also available. Cytometric instrumentation is expensive and beyond the budget of most individual researchers. The CCF is easily accessible and is responsible for procurement, daily calibration, maintenance and repair of all instrumentation. CCF personnel have a combined 82 years of flow cytometry and 46 years of cytokine measurement experience. Expertise is available to investigators through one-on-one consultation, classes, a website, poster presentations, and open houses. Ms. Schmid and Dr. Uittenbogaart also work with researchers to adapt or develop assays to enable investigators to pursue research questions in novel ways. CCF services help researchers with varying amounts of experience in cytometry to perform high quality assays with minimal time expenditure. Workshops, seminars, and utilization of new assays ensure that researchers remain on the cutting edge of cytometry without investing their own funds or staff time on keeping current in the latest advances. During the last funding period a total of 250 investigators used the CCF; 40 of those were CFAR/AIDS Institute members representing 38% of use. This documents the role of the CCF in enhancing HIV research at UCLA with its potential contribution to improving the health of HIV-infected patients.

加州大学洛杉矶分校细胞计数核心设施（CCF），一个校园范围内的资源，提供最先进的仪器 和专业知识，以负担得起的成本，加州大学洛杉矶分校的研究人员需要流式细胞仪和ELISpot图像细胞术。在 1988年，琼森综合癌症中心建立了资源，自1991年以来， 艾滋病研究提供了额外支助。自2000年以来，杰米森博士，一个有成就的细胞 免疫学家，指导CCF，并可供用户咨询。施密德女士， 自1988年以来，负责CCF的日常运作，并协助Jamieson博士进行长期的 规划Schmid女士是一位流式细胞仪专家，以其流式细胞术的发展而闻名 方法和她在建立细胞分选生物安全指南方面的关键作用。自2001年以来， 在著名细胞免疫学家Uittenbogaart博士的指导下提供ELISpot检测服务， 在细胞因子测量方面拥有丰富的专业知识。CCF具有单激光FACScan、双激光 FACSCalibur，一个三激光BD LSR I，一个改进的，双激光，五色FACScan，三，三激光分选机， autoMACS磁分离器和离线数据分析计算机。免疫斑点分析仪，三， 此外还提供独立式分析仪和五个生物安全柜。细胞计数仪器是 昂贵的，超出了大多数研究人员的预算。CCF很容易获得， 负责所有仪器仪表的采购、日常校准、维护和维修。CCF人员 拥有82年流式细胞术和46年细胞因子测量经验。专门知识 通过一对一的咨询、课程、网站、海报展示和 开放参观Schmid女士和Uittenbogaart博士还与研究人员合作，调整或开发检测方法， 使研究人员能够以新颖的方式追求研究问题。CCF服务帮助研究人员 不同数量的细胞计数经验，以最少的时间消耗进行高质量的分析。 讲习班、研讨会和新检测方法的利用确保研究人员保持在 他们不需要投入自己的资金或工作人员的时间来保持最新的进展。期间 在上一个资助期间，共有250名调查人员使用CCF;其中40人是CFAR/艾滋病研究所 占使用量的38%。该文件记录了CCF在加强UCLA艾滋病毒研究方面的作用 它可能有助于改善艾滋病毒感染者的健康。