Genetic Linkage Mapping in Rhesus Monkeys

恒河猴的遗传连锁图谱

• 批准号: 7983791
• 负责人: JEFFREY A. ROGERS
• 金额: $ 24.75万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7983791
• 关键词: AIDS vaccine development; Acquired Immunodeficiency Syndrome; Alcoholism; Animal Model; Animals; Area; Arts; Biology; Biomedical Research; Budgets; Chromosome Mapping; Collaborations; Communities; DNA Sequence; Data; Data Linkages; Databases; Development; Diabetes Mellitus; Disease; Generations; Genes; Genetic; Genetic Markers; Genetic Polymorphism; Genome; Genomics; Genotype; Goals; HIV Infections; Health; Human; Immunology; Informatics; Investigation; Laboratories; Leadership; Location; Macaca; Macaca mulatta; Maps; Methods; Microsatellite Repeats; Monoclonal Antibody R24; Neurobiology; Neurosciences; Obesity; Pathogenesis; Pharmacology; Physical Map of the Human Genome; Positioning Attribute; Primates; Psychology, Other; Quantitative Trait Loci; Range; Reproductive Biology; Request for Applications; Research; Research Personnel; Research Project Grants; Resources; Science; Screening procedure; United States National Institutes of Health; autosome; base; bone; density; design; developmental psychology; genetic analysis; genetic linkage analysis; genetic pedigree; genome sequencing; human disease; improved; nonhuman primate; novel; novel strategies; physical mapping; programs; tool; trait

The goal of this competitive renewal is to increase the value of rhesus macaques (Macaca mulatto) for genetic and genomic research by continuing development of a highly informative genetic linkage map for this species. Rhesus monkeys are the most widely used nonhuman primate in biomedical research. This species is already an important model organism for studies of neurobiology, immunology, HIV infection and AIDS, pharmacology, diabetes and obesity, bone biology, alcoholism, developmental psychology and other significant human health problems. Improved research tools and information related to genetics and genomics will make this species even more valuable for analyses of the genetic basis of various human diseases. During the first four years of this project, an initial genetic linkage map was produced that consists of 241 human microsatellite loci genotyped and analyzed in more than 800 pedigreed rhesus monkeys. This initial map covers all 20 rhesus autosomes, and the average spacing between markers is approximately 9.3 centiMorgans. However, the value of this map is limited by the lack of genetic markers in some chromo- somal regions, and the low heterozygosity of loci in others. In this renewal application, support is requested to add 300 additional microsatellite polymorphisms to this initial rhesus linkage map. This will improve the density, coverage and scientific value of the rhesus linkage map. Support is also requested to establish a collaboration with the Baylor Human Genome Sequencing Center in order to integrate the growing genetic linkage map with the whole genome DNA sequence that BHGSC is generating for this species. Integration of genetic linkage data with physical mapping and sequence data will benefit both projects and make these resources and this species more useful for many different genetic analyses. Finally, this project will establish a website designed to give the research community easy access to up-to-date rhesus linkage mapping data, including detailed information about the microsatellite polymorphisms discovered through this study. A more informative linkage map for this species will significantly increase the opportunity for and statistical power of whole genome linkage screens (i.e. QTL mapping analyses) intended to locate functional genes that influence significant traits related to human health and disease.

这次竞争性更新的目标是提高恒河猴（Macaca mulatto）的价值。 通过继续开发高度信息化的遗传连锁图， 这个物种。恒河猴是生物医学研究中应用最广泛的非人灵长类动物。这 物种已经是神经生物学、免疫学、艾滋病毒感染和 艾滋病、药理学、糖尿病和肥胖症、骨生物学、酗酒、发展心理学和其他 严重的人类健康问题。改进与遗传学有关的研究工具和信息， 基因组学将使这一物种在分析各种人类遗传基础方面更具价值。 疾病在该项目的前四年中，制作了一个初步的遗传连锁图， 对800多只纯种恒河猴的241个人类微卫星位点进行了基因分型和分析。这 初步图谱覆盖所有20个恒河猴常染色体，标记之间的平均间距约为9.3 厘摩根然而，这张图的价值受到了一些染色体遗传标记缺乏的限制， 染色体区域，和其他基因座的低杂合性。在此更新申请中， 在这个恒河猴的连锁图谱上增加300个额外的微卫星多态性。这将改善 恒河猴连锁图的密度、覆盖率和科学价值。还要求提供支助， 与贝勒人类基因组测序中心合作，以整合不断增长的基因 BHGSC正在为该物种生成的全基因组DNA序列的连锁图。一体化 将遗传连锁数据与物理作图和序列数据相结合将使这两个项目受益， 资源和本种更有用的许多不同的遗传分析。最后，该项目将建立 一个网站，旨在使研究界容易获得最新的恒河猴连锁图谱数据， 包括通过这项研究发现的微卫星多态性的详细信息。一 更多的信息连锁图，这一物种将显着增加的机会和统计 全基因组连锁筛选（即QTL作图分析）的能力，旨在定位功能基因， 影响与人类健康和疾病相关的重要特征。