GENETIC LINKAGE MAPPING IN RHESUS MACAQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 恒河猴是生物医学研究中最常用的非人类灵长类动物。该物种被用作多种人类疾病的模式生物，人们普遍认为，恒河猴的基因组信息，包括遗传连锁图谱，将成为大量生物医学研究的重要资源。该项目正在开发一种资源(遗传连锁图谱)，这将使恒河猴在各种研究项目中更有价值，特别是分析常见人类疾病的遗传基础。其目标是制作一张恒河猴基因组的连锁图，标记之间的平均间距不超过5厘米。用于定位恒河猴染色体的标记(多态)将包括两种类型：a)已在人类基因组中定位的人类微卫星座位，b)使用恒河猴全基因组DNA序列识别和表征的新的恒河猴来源的微卫星。使用大量的人类基因座来定位恒河猴染色体将产生一个易于与人类基因组图谱进行比较的恒河猴遗传图谱，而添加恒河猴衍生的基因座将增加标记密度，从而增加信息含量。我们已经确定了几个适合进行连锁分析的恒河猴多世代家族。该项目使用了1000多只恒河猴，包括来自俄勒冈州国家灵长类动物研究中心殖民地和SNPRC殖民地的动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rhesus macaques are the most commonly used nonhuman primate in biomedical research. This species is used as a model organism for a wide range of human diseases, and it is widely acknowledged that genomic information, including genetic linkage maps, for rhesus monkeys would constitute an important resource for a large number of biomedical studies. This project is developing a resource (a genetic linkage map) that will make rhesus monkeys more valuable for a wide variety of research projects, especially the analysis of the genetic basis of common human diseases. The objective is to produce a linkage map of the rhesus genome with average spacing among markers of less than 5 centiMorgans. The markers (polymorphisms) to be used to map the rhesus chromosomes will consist of two types: a) human microsatellite loci already mapped in the humane genome, and b) novel rhesus-derived microsatellites identified and characterized using the rhesus whole genome DNA sequence. Using a significant number of human loci to map rhesus chromosomes will produce a rhesus genetic map that can be readily compared with the human genome map, while adding rhesus-derived loci will increase marker density and thus the information content. We have identified several multi-generation families of rhesus monkeys that are suitable for linkage analysis. More than 1000 rhesus monkeys are used for this project, including animals from the Oregon National Primate Research Center colony and the SNPRC colony.