GENETIC LINKAGE MAPPING IN RHESUS MACAQUES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 恒河猴是生物医学研究中最常用的非人灵长类动物。 这一物种被用作一系列人类疾病的模式生物，人们普遍认为，恒河猴的基因组信息，包括遗传连锁图，将构成大量生物医学研究的重要资源。 该项目正在开发一种资源（遗传连锁图），使恒河猴在各种研究项目中，特别是在分析人类常见疾病的遗传基础方面更有价值。 目的是建立一个平均间隔小于5厘摩的恒河猴基因组连锁图。 用于定位恒河猴染色体的标记（多态性）将由两种类型组成：a）已经在人类基因组中定位的人类微卫星基因座，和B）使用恒河猴全基因组DNA序列鉴定和表征的新的恒河猴衍生的微卫星。 使用大量的人类基因座来绘制恒河猴染色体将产生恒河猴遗传图，其可以容易地与人类基因组图进行比较，而添加恒河猴衍生的基因座将增加标记密度，从而增加信息含量。 我们已经确定了几个多代家庭的恒河猴，适合连锁分析。 超过1000只恒河猴被用于这个项目，包括来自俄勒冈州国家灵长类动物研究中心和SNPRC的动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rhesus macaques are the most commonly used nonhuman primate in biomedical research. This species is used as a model organism for a wide range of human diseases, and it is widely acknowledged that genomic information, including genetic linkage maps, for rhesus monkeys would constitute an important resource for a large number of biomedical studies. This project is developing a resource (a genetic linkage map) that will make rhesus monkeys more valuable for a wide variety of research projects, especially the analysis of the genetic basis of common human diseases. The objective is to produce a linkage map of the rhesus genome with average spacing among markers of less than 5 centiMorgans. The markers (polymorphisms) to be used to map the rhesus chromosomes will consist of two types: a) human microsatellite loci already mapped in the humane genome, and b) novel rhesus-derived microsatellites identified and characterized using the rhesus whole genome DNA sequence. Using a significant number of human loci to map rhesus chromosomes will produce a rhesus genetic map that can be readily compared with the human genome map, while adding rhesus-derived loci will increase marker density and thus the information content. We have identified several multi-generation families of rhesus monkeys that are suitable for linkage analysis. More than 1000 rhesus monkeys are used for this project, including animals from the Oregon National Primate Research Center colony and the SNPRC colony.