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中文摘要
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核心 C:分析 分析核心,在 Nouri Neamati 博士的指导下,助理教授 制药科学,将处理、存储和分析所有组织和适当的样本 用于本计划项目的研究目的。组织以及液体、标本包括 将在需要时收集血液、血清和尿液,供个别研究人员提出假设 驱动的转化研究。分析核心将提供以下服务: 适当的: 1) 使用SuperArray平台对100个高度相关的定制基因进行宏阵列分析。 2) 选定基因的实时PCR。 3) 质谱分析,包括对选定样品的 MALDI 和 MS/MS 分析。 4) 通过带有质量选择检测器的气相色谱仪分析黄体酮和代谢物 利用电子碰撞电离,以及 5) 通过以下方法对孕酮和代谢物进行定量分析 放射免疫测定。
英文摘要
Core C: Analytic The Analytical Core, under the direction of Dr. Nouri Neamati, Assistant Professor of Pharmaceutical Sciences, will process, bank, and analyze all tissues and appropriate samples for research purposes for use in this Program Project. The tissues as well as fluid, specimens including blood, serum, and urine will be collected where needed for individual investigators with hypothesis driven translational research. The Analytical Core will provide the following services where appropriate: 1) Macroarray analysis of 100 highly relevant customized genes using SuperArray platform. 2) Real-Time PCR for selected genes. 3) Mass Spectrometry including MALDI and MS/MS analysis for selected samples. 4) Progesterone and metabolite analysis by Gas Chromatograph with Mass Selective Detector utilizing Electron Impact ionization, and 5) Quantitative analysis of progesterone and metabolites by radioimmunoassays.
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