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CORE--ANALYTIC

核心--分析

基本信息

批准号：
7864197
负责人：
NOURI NEAMATI
金额：
$ 20.93万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7864197
关键词：
Alzheimer&apos s Disease Alzheimer&apos s disease model Animals Bioinformatics Blood Brain Brain region Cataloging Catalogs Cluster Analysis Custom Discrimination Electrons Ensure Future Gases Gene Expression Genes Hippocampus (Brain)Hormone Antagonists Hormones Individual Liquid substance Mass Spectrum Analysis Menopause Modeling Mus Pathway interactions Pattern Pattern Recognition Perimenopause Pharmacologic Substance Plasma Process Progesterone Proteins RNA Processing Radioimmunoassay Rattus Research Research Personnel Reverse Transcriptase Polymerase Chain Reaction Sampling Science Serum Services Solutions Specimen Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Time Tissue Banking Tissue Banks Tissue Sample Tissues Translational Research Urine Uterus aging brain brain tissue computational chemistry detector hormone metabolism inhibitor/antagonist ionization member professor programs research study

项目摘要

Core C: Analytic The Analytical Core, under the direction of Dr. Nouri Neamati, Assistant Professor of Pharmaceutical Sciences, will process, bank, and analyze all tissues and appropriate samples for research purposes for use in this Program Project. The tissues as well as fluid, specimens including blood, serum, and urine will be collected where needed for individual investigators with hypothesis driven translational research. The Analytical Core will provide the following services where appropriate: 1) Macroarray analysis of 100 highly relevant customized genes using SuperArray platform. 2) Real-Time PCR for selected genes. 3) Mass Spectrometry including MALDI and MS/MS analysis for selected samples. 4) Progesterone and metabolite analysis by Gas Chromatograph with Mass Selective Detector utilizing Electron Impact ionization, and 5) Quantitative analysis of progesterone and metabolites by radioimmunoassays.

核心C：分析分析核心，在Nouri Neamati博士的指导下，助理教授制药科学，将处理，银行，和分析所有组织和适当的样本本计划项目中使用的研究目的。组织和液体，标本包括血液、血清和尿液将在需要的地方收集，供个别调查人员进行假设推动翻译研究。分析核心将在以下情况下提供服务适当的： 1)利用SuperArray平台对100个高度相关的定制基因进行大数组分析。 2)选定基因的实时荧光定量聚合酶链式反应。 3)选择样品进行质谱分析，包括MALDI和MS/MS分析。 4)孕酮和代谢物的气相色谱-质量选择检测器分析利用电子碰撞电离和5)黄体酮及其代谢物的定量分析。放射免疫分析。