CORE--ANALYTIC

核心--分析

• 批准号: 7626295
• 负责人: NOURI NEAMATI
• 金额: $ 20.37万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7626295
• 关键词: Alzheimer' s disease model; Animals; Bioinformatics; Blood; Brain; Brain region; Cataloging; Catalogs; Cluster Analysis; Custom; Discrimination; Electrons; Ensure; Future; Gases; Gene Expression; Genes; Hippocampus (Brain); Hormone Antagonists; Hormones; Individual; Liquid substance; Mass Spectrum Analysis; Menopause; Modeling; Mus; Numbers; Pathway interactions; Pattern; Pattern Recognition; Perimenopause; Pharmacologic Substance; Plasma; Polymerase Chain Reaction; Process; Progesterone; Proteins; Purpose; RNA Processing; Radioimmunoassay; Rattus; Research; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Science; Serum; Services; Solutions; Specimen; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time; Tissue Banking; Tissue Sample; Tissues; Translational Research; Urine; Uterus; brain tissue; computational chemistry; detector; hormone metabolism; inhibitor/antagonist; ionization; member; professor; programs; research study

Core C: Analytic The Analytical Core, under the direction of Dr. Nouri Neamati, Assistant Professor of Pharmaceutical Sciences, will process, bank, and analyze all tissues and appropriate samples for research purposes for use in this Program Project. The tissues as well as fluid, specimens including blood, serum, and urine will be collected where needed for individual investigators with hypothesis driven translational research. The Analytical Core will provide the following services where appropriate: 1) Macroarray analysis of 100 highly relevant customized genes using SuperArray platform. 2) Real-Time PCR for selected genes. 3) Mass Spectrometry including MALDI and MS/MS analysis for selected samples. 4) Progesterone and metabolite analysis by Gas Chromatograph with Mass Selective Detector utilizing Electron Impact ionization, and 5) Quantitative analysis of progesterone and metabolites by radioimmunoassays.

核心C：分析 分析核心，在大学助理教授Nouri Neamati博士的指导下 制药科学部将处理、储存和分析所有组织和适当样本， 用于本项目的研究目的。组织和液体，标本包括 将在需要时采集血液、血清和尿液，供个体研究者假设 推动翻译研究。分析核心将提供以下服务， 适当的： 1)使用SuperArray平台对100个高度相关的定制基因进行宏阵列分析。 2)选择基因的实时PCR。 3)质谱法，包括选定样品的MALDI和MS/MS分析。 4)用气相色谱-质量选择检测器分析孕酮和代谢产物 利用电子碰撞电离，和5）通过 放射免疫测定