Topical Microbicide Against SIV and Chlamydia

描述(由申请人提供)：本申请的主要目的是测试一种潜在的杀微生物剂的有效性，以防止艾滋病毒传播给妇女。迫切需要这样一种有效的媒介，以应对全球妇女艾滋病毒新感染人数普遍上升的现象。基于有希望的体外初步结果，这项应用的具体目的是在体内测试单月桂酸甘油(GML)防止猴免疫缺陷病毒(SIV)经阴道传播给恒河猴的有效性。恒河猴是一种高度相关的非人类灵长类动物，HIV的性黏膜传播模型。经治疗或对照的猴子将被感染，活检和尸检样本将通过免疫组织化学和原位杂交方法以及血液中的病毒载量进行分析，以评估GML在预防传播或改变病毒在宫颈阴道部和 淋巴组织。

DESCRIPTION (provided by applicant): This application's major objective is to test the efficacy of a potential microbicidal agent to prevent transmission of HIV to women. Such an effective agent is urgently needed to combat the predominant rise globally of new HIV infections in women. Based on promising preliminary results in vitro, the specific aim of this application is to test in vivo the efficacy of glycerol monolaurate (GML) to prevent intravaginal transmission of simian immunodeficiency virus (SIV) to rhesus macaques, a highly relevant nonhuman primate model of sexual mucosal transmission of HIV. Treated or control monkeys will be infected and biopsy and necropsy specimens will analyzed by immunohistochemical and in situ hybridization methods and viral loads in blood to assess the efficacy of GML in preventing transmission or altering viral replication in cervicovaginal and lymphatic tissues.