Impact of Extraordinarily Large Numbers of SIV-specific CD8 T Cells on Vaginal Tr

描述(由申请人提供)： 需要一种有效的疫苗来预防艾滋病毒-1的传播。这项提议将在艾滋病毒-1向女性传播的SIV-恒河猴模型中测试这样一个概念，即由新的异源Prime-Boost策略产生的非常大量的SIV特异性CTL可以通过消除进入入口处的感染细胞的小创建者群体来提供保护。用流式细胞术和原位四聚体染色和杂交的方法在相关组织部位记录大量的病毒特异性CD8T细胞及其与SIV感染细胞的相互作用。这一战略的成功应该会使疫苗设计成为可能。迫切需要一种有效的疫苗来预防艾滋病毒-1。在这项建议中，描述了一种开发这种疫苗的新方法，并在SIV-恒河猴向妇女传播HIV-1的模型中测试了其有效性。

DESCRIPTION (provided by applicant): An effective vaccine is needed for prevention of HIV-1 transmission. This proposal will test in the SIV-rhesus macaque model of HIV-1 transmission to women the concept that extraordinarily large numbers of SIV-specific CTLs generated by a novel heterologous prime-boost strategy can confer protection by eliminating small founder populations of infected cells at the portal of entry. Flow cytometric and in situ methods of in situ tetramer staining and hybridization will be used to document at relevant tissue sites the large numbers of virus specific CD8 T cells and their interactions with SIV-infected cells. Success of this strategy should enable vaccine design. An effective vaccine to prevent HIV-1 is urgently needed. In this proposal a novel approach to developing such a vaccine is described with tests of its efficacy in the SIV-rhesus macaque model of HIV-1 transmission to women.