Impact of Extraordinarily Large Numbers of SIV-specific CD8 T Cells on Vaginal Tr

大量 SIV 特异性 CD8 T 细胞对阴道 Tr 的影响

• 批准号: 8293429
• 负责人: ASHLEY T. HAASE
• 金额: $ 73.64万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8293429
• 关键词: Acquired Immunodeficiency Syndrome; Animals; CD4 Positive T Lymphocytes; CD8B1 gene; Cell Communication; Cells; Cervical; Development; Distal; Dose; Founder Generation; Gagging; HIV-1; Host Defense; Immune response; Immunodominant Epitopes; In Situ; In Situ Hybridization; Infection; Lymphoid Tissue; Macaca mulatta; Methods; Modeling; Pathology; Prevention; Research; SIV; Site; Staining method; Stains; Systemic infection; T cell response; T-Cell Depletion; T-Lymphocyte; Technology; Testing; Tissues; Vaccination; Vaccine Design; Vaccines; Vagina; Viral Load result; Virus; Woman; experience; nonhuman primate; novel; novel strategies; novel vaccines; pandemic disease; prevent; response; success; tool development; transmission process; vaginal transmission; vector

DESCRIPTION (provided by applicant): An effective vaccine is needed for prevention of HIV-1 transmission. This proposal will test in the SIV-rhesus macaque model of HIV-1 transmission to women the concept that extraordinarily large numbers of SIV-specific CTLs generated by a novel heterologous prime-boost strategy can confer protection by eliminating small founder populations of infected cells at the portal of entry. Flow cytometric and in situ methods of in situ tetramer staining and hybridization will be used to document at relevant tissue sites the large numbers of virus specific CD8 T cells and their interactions with SIV-infected cells. Success of this strategy should enable vaccine design. An effective vaccine to prevent HIV-1 is urgently needed. In this proposal a novel approach to developing such a vaccine is described with tests of its efficacy in the SIV-rhesus macaque model of HIV-1 transmission to women.

描述（由申请人提供）： 需要一种有效的疫苗来预防HIV-1的传播。该提案将在HIV-1传播给女性的SIV-恒河猴模型中测试以下概念：由新型异源引发-加强策略产生的非常大量的SIV特异性CTL可以通过消除入口处感染细胞的小创始人群体来提供保护。将使用流式细胞术和原位四聚体染色和杂交的原位方法来记录相关组织部位的大量病毒特异性CD 8 T细胞及其与SIV感染细胞的相互作用。这一策略的成功将使疫苗设计成为可能。 迫切需要一种有效的疫苗来预防HIV-1。在这项提案中，描述了一种开发这种疫苗的新方法，并在HIV-1传播给女性的SIV-恒河猴模型中对其效力进行了测试。