Small Animal Phenotyping Core

小动物表型核心

• 批准号: 7333102
• 负责人: BARBARA A GOWER
• 金额: $ 10.87万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7333102
• 关键词: Abdomen; Adipose tissue; Amino Acids; Animals; Arts; Binding Proteins; Biological; Body Composition; Body Water; Body fat; C-Peptide; Child; Chronic; Clinical; Clinical Nutrition; Data; Diabetes Mellitus; Dual-Energy X-Ray Absorptiometry; Elements; Endocrine; Energy Metabolism; Estradiol; Fatty acid glycerol esters; Funding; GNAI2 gene; Glucagon; Glucose; Gonadotropins; Hormones; Human; In Vitro; Inflammation; Instruction; Insulin; Insulin-Like Growth Factor I; Interleukin 6 Receptor; Intra-abdominal; Invasive; Isotopes; Label; Leptin; Life; Lipids; Liquid substance; Mass Spectrum Analysis; Measurement; Metabolic Bone Diseases; Metabolic Diseases; Metabolism; Methodology; Modeling; OGTT; Obesity; Phenotype; Research Personnel; Research Project Grants; Research Training; Resources; Risk; Sampling; Scanning; Services; Site; Somatomedins; Specimen; Students; System; Testing; Testosterone; Tissues; Training; Validation; Water; X-Ray Computed Tomography; adiponectin; body density; bone; cost; cysteine rich protein; cytokine; data modeling; glucagon-like peptide 1; glucose production; in vivo; indexing; insulin secretion; insulin sensitivity; intravenous glucose tolerance test; metabolic abnormality assessment; method development; protein degradation; reproductive; steroid hormone; subcutaneous; tissue culture

The Metabolism Core is a state-of-the art facility for assessing human energy expenditure, body composition, and risk for chronic metabolic disease. It provides expertise, resources, and training in: ¿ Energy expenditure (free-living energy expenditure by doubly-labeled water and isotope-ratio mass spectrometry); ¿ Body composition and fat distribution [(underwater weighing and BodPod for measurement of whole body density; dual-energy X-ray absorptiometry (DXA) for measurement of regional and whole-body bone, fat, and soft lean tissue; isotope dilution for assessment of total body water; multi-compartment models of body composition; analysis of regional body composition (intra-abdominal and subcutaneous abdominal adipose tissue from computed tomography scans)]; ¿ Insulin sensitivity testing (frequently-sampled, intravenous, glucose tolerance test with minimal modeling); oral glucose tolerance test with analysis of insulin and glucose; ¿ Analysis of glucose, lipids, and hormones (including obesity-related hormones, e.g., leptin, adiponectin; diabetes-related hormones, e.g., insulin, C-peptide, glucagon, GLP-1, GIP; a reproductive-endocrine panel, e.g., estradiol, testosterone, other steroid hormones, gonadotropins; elements of the IGF system, e.g., IGF-1 and its binding proteins; cytokines and markers of inflammation, e.g., TNF-a and its receptors, IL-6, CRP); ¿ Analysis of isotopically-labeled glucose and amino acids by GC/MS for use in in vivo metabolic studies (e.g., endogenous glucose production, protein turnover). Since inception in 2000, Core A has supported 65 investigators and 102 research projects. Current core use includes 24 investigators from 13 departments/divisions across DAB, and seven investigators from outside of DAB. These investigators have a total of 47 funded projects that use Core services. Of these projects, 38 (81%) are federally-funded. Two CNRU P/F studies presently use the Metabolism Core. New Methods Development includes, 1) the validation of a non-invasive test for insulin sensitivity in children using a mixed-meal, and, 2) implementation of methodology for assessing insulin secretion and clearance using data resulting from a mixed-meal test.

代谢核心是一种最先进的设备，用于评估人体能量消耗，身体成分，