Small Animal Phenotyping Core

代谢核心是一个国家的最先进的设施，用于评估人类的能量消耗，身体成分， 和患慢性代谢疾病的风险。它提供以下方面的专业知识、资源和培训： 能量消耗（通过双标记水和同位素比质量计算的自由生活能量消耗 光谱法）; <$身体成分和脂肪分布[（水下称重和BodPod用于测量整体 身体密度;测量局部和全身的双能X线吸收测定法 骨、脂肪和瘦肉软组织;评估全身水分的同位素稀释;多室 身体成分模型;局部身体成分分析（腹内和皮下 腹部脂肪组织，来自计算机断层扫描）]; <$胰岛素敏感性试验（频繁采样，静脉注射，葡萄糖耐量试验， 口服葡萄糖耐量试验，分析胰岛素和葡萄糖; 葡萄糖、脂质和激素（包括肥胖相关激素，例如，瘦素、脂联素; 糖尿病相关激素，例如，胰岛素、C肽、胰高血糖素、GLP-1、GIP;生殖内分泌 面板，例如，雌二醇、睾酮、其他类固醇激素、促性腺激素; IGF系统的要素， 例如，在一个实施例中，IGF-1及其结合蛋白;细胞因子和炎症标志物，例如，TNF-α及其 受体、IL-6、CRP）; 通过GC/MS分析同位素标记的葡萄糖和氨基酸，用于体内代谢研究 (e.g.,内源性葡萄糖产生、蛋白质周转）。 自2000年成立以来，核心A已经支持了65名调查员和102个研究项目。当前核心用途 包括来自DAB 13个部门/司的24名调查员和来自外部的7名调查员 的DAB。这些调查人员共有47个使用核心服务的资助项目。在这些项目中，38个 (81%）由联邦政府资助。两项CNRU P/F研究目前使用代谢核心。 新方法的开发包括：1）儿童胰岛素敏感性的非侵入性测试的验证 使用混合膳食，以及2）实施用于评估胰岛素分泌和清除的方法 使用混合膳食测试的数据。

The Metabolism Core is a state-of-the art facility for assessing human energy expenditure, body composition, and risk for chronic metabolic disease. It provides expertise, resources, and training in: ¿ Energy expenditure (free-living energy expenditure by doubly-labeled water and isotope-ratio mass spectrometry); ¿ Body composition and fat distribution [(underwater weighing and BodPod for measurement of whole body density; dual-energy X-ray absorptiometry (DXA) for measurement of regional and whole-body bone, fat, and soft lean tissue; isotope dilution for assessment of total body water; multi-compartment models of body composition; analysis of regional body composition (intra-abdominal and subcutaneous abdominal adipose tissue from computed tomography scans)]; ¿ Insulin sensitivity testing (frequently-sampled, intravenous, glucose tolerance test with minimal modeling); oral glucose tolerance test with analysis of insulin and glucose; ¿ Analysis of glucose, lipids, and hormones (including obesity-related hormones, e.g., leptin, adiponectin; diabetes-related hormones, e.g., insulin, C-peptide, glucagon, GLP-1, GIP; a reproductive-endocrine panel, e.g., estradiol, testosterone, other steroid hormones, gonadotropins; elements of the IGF system, e.g., IGF-1 and its binding proteins; cytokines and markers of inflammation, e.g., TNF-a and its receptors, IL-6, CRP); ¿ Analysis of isotopically-labeled glucose and amino acids by GC/MS for use in in vivo metabolic studies (e.g., endogenous glucose production, protein turnover). Since inception in 2000, Core A has supported 65 investigators and 102 research projects. Current core use includes 24 investigators from 13 departments/divisions across DAB, and seven investigators from outside of DAB. These investigators have a total of 47 funded projects that use Core services. Of these projects, 38 (81%) are federally-funded. Two CNRU P/F studies presently use the Metabolism Core. New Methods Development includes, 1) the validation of a non-invasive test for insulin sensitivity in children using a mixed-meal, and, 2) implementation of methodology for assessing insulin secretion and clearance using data resulting from a mixed-meal test.