CORE--MOLECULAR PATHOLOGY FACILITY

描述(由申请人提供)：分子病理学设施核心提供支持， 为细胞、组织和生物体液的组织学和病理学评估的所有方面提供咨询和设备。它是外源生物对动物和细胞培养系统影响的形态分析和诊断的重点。该中心的作用是充当该中心研究活动的组织学/病理学分支。具体目标是：1)向中心成员提供准备高质量组织样本的设施，以支持新的和正在进行的研究倡议；2)在组织样本的病理评估方面提供专业知识和咨询，并协作协助 中心成员致力于开发/应用现代分子病理学技术，用于外来生物诱发损伤的机制研究；3)使用高性能临床酶分析仪对样本进行大规模、迭代的酶分析；4)为研究人员、工作人员和学生提供各种技术和组织形态解释方面的培训；5)预测分子病理学新方法的需求，并建立其使用方面的专门知识。核心有两个设施，提供互补的功能。中心实验室位于神经毒理学实验室，专门用于小动物手术和尸检、组织学准备、细胞培养、光学和电子显微镜、定量图像分析/形态计量学、酶分析和动物收容。会议室配备了五头显微镜和视频显示器，方便咨询和培训。第二个设施位于EOSHI大楼，包含用于显微镜和样品制备的附加仪器。此外，一台激光捕获显微镜(LCM)位于EOHSI大楼附近的癌症研究实验室。

Description (provided by applicant): The Molecular Pathology Facility Core provides support, consultation and equipment access for all facets of histological and pathological assessment of cells, tissues and biological fluids. It serves as the focus of morphological analysis and diagnosis of xenobiotic effects on animals and cell culture systems. The role of the core is to act as the histology/pathology arm for the Center research activities. The specific objectives are to, 1) make available to members of the Center a facility for the preparation of high-quality histological samples in support of new and ongoing research initiatives; 2) provide expertise and consultation in the pathological assessment of histological samples and to collaboratively assist Center members in the development/application of contemporary techniques in molecular pathology for mechanistic studies of xenobiotic-induced injury; 3) provide large-scale, iterative enzymatic analyses of samples using a high-capacity clinical enzyme analyzer; 4) provide training for investigators, staff and students in various technologies, and in the interpretation of tissue morphology; and 5) anticipate the need for emerging methodologies in molecular pathology and establish expertise in their use. The core has two facilities, which provide complementary functions. The central laboratory is located in the Neurotoxicology Laboratories and has dedicated space for small animal surgery and necropsy, histological preparation, cell culture, light and electron microscopy, quantitative image analysis/morphometry, enzyme analysis and animal housing. A conference room is equipped with a five-headed microscopy and video display facilitating consultations and training. The second facility is located in the EOSHI building, and contains additional instrumentation for microscopy and sample preparation. Additionally, a laser-capture microscope (LCM) is located in the Laboratory for Cancer Research adjacent to the EOHSI building.