CORE--MOLECULAR PATHOLOGY FACILITY

分子病理学中心(MPFC)成立于1996年 由于细胞酶学设施核心和 组织学实验室。MPFC目前执行各种各样的 评估，包括光学和电子显微镜、图像分析、 免疫细胞化学、原位杂交印迹法(Northern、Western 和Southern)，以及酶分析。MPFC有两个设施：中环 实验室位于基尔默戈登路的神经毒理实验室 校园；较小的卫星设施位于132、134和136室 布希校区的EOHSI大楼。 强制性公积金计划委员会的具体目标是：a)向成员提供 EHS中心是准备高质量组织学的设施 支持新的和正在进行的研究举措的样本；b)提供 组织学病理评估方面的专业知识和咨询 样本，并协作协助中心成员开发和 现代技术在分子病理学中的应用(例如，免疫电子学 显微镜、荧光原位杂交)用于机理研究 异种生物诱导的损伤；c)提供大规模、迭代的酶 使用FARA II离心酶分析仪对样品进行分析；d) 为调查人员、学生和技术人员提供各种方面的培训 组织形态的技术和解释；以及e)预测 需要新兴的病理学方法，并在其 使用。 MPFC的使命和目标是促进两国之间的互动研究 中心的成员，而不是作为一个纯粹的服务实验室。 适度的费用由MPFC的业务预算承担。复发性 较大规模的研究费用由用户承担？S说。这一核心具有 得到了广泛的利用。在过去的四年里，14,500个街区 已制备并切片用于光学显微镜，并对1400个样品进行了 用于电子显微镜检查。所有Research内核都使用了MPFC。这个 使用最多的是核I和核III，核II和核IV的使用率较低。 MPFC在培训人员方面非常积极：15名毕业生 每年对学生进行组织学技术培训，8名学生或 博士后接受了使用FARA分析仪，EM， 和分子病理学技术。它也在夏季的几个月里使用。 与NIH赞助的高中少数民族学徒计划相结合 在校学生。 一系列关于未来方向的规定已经实施。 两间化验所合并后成立MPFC。鲁尔博士 将保留对组织学部分的全面责任 设施核心职能，而Lowndes博士将保留以下职责 酶学和免疫化学活性。日常运作将是 由一名技术人员在鲁尔博士的直接监督下承担。 核心升级包括以下几个方面：镜像升级 将寻求分析/形态系统，以允许更多地使用桌面 组织学图像、免疫化学和图形的出版技术。 利用EHS中心现有的组织病理学专业知识， 已经成立了毒性病理学用户小组，以审查幻灯片和 讨论与病理相关的问题。今后对文书的升级包括： 1)对FARA II离心式分析仪进行了7年的软件升级 2)Preage CV-6图像分析仪的软件升级。

Molecular Pathology Facility Core (MPFC) was established in 1996 as a result of the merger of the Cellular Enzymology Facility Core and the Histology Laboratory. The MPFC currently performs a wide variety of evaluations, including light- and electron-microscopy, image analysis, immunocytochemistry, in situ hybridization blotting methods (Northern, Western and Southern), and enzyme analysis. The MPFC has two facilities: the central laboratory is located in the Neurotoxicology Laboratory at Gordon Road, Kilmer Campus; and a smaller satellite facility is located in Rooms 132, 134 and 136 of the EOHSI building on Busch Campus. The specific objectives of the MPFC are: a) to make available to members of the EHS Center a facility for the preparation of high-quality histological samples in support of new and ongoing research initiatives; b) to provide expertise and consultation in the pathological assessment of histological samples, and collaboratively to assist Center members in the development and use of contemporary techniques in molecular pathology (e.g., immunoelectron microscopy, fluorescence in situ hybridization) for mechanistic studies of xenobiotic-induced injury; c) to provide large scale, iterative enzymatic analyses of samples using the FARA II centrifugal enzyme analyzer; d) to provide training for investigators, students and technical staff in various technologies and interpretation of tissue morphology; and e) to anticipate the need for emerging pathology methodologies and to establish expertise in their use. The mission and goal of the MPFC is to foster interactive research between members of the Center rather than functioning as a purely service laboratory. Modest costs are absorbed by the operating budget of the MPFC. Recurrent costs for larger studies are charged back to the user?s grants. This Core has been utilized extensively. During the past four years, 14,500 blocks have been prepared and sectioned for light microscopy, and 1,400 samples were embedded for electron microscopy. All Research Cores used the MPFC. The heaviest users were Cores I and III, with Cores II and IV using less heavily. The MPFC has been extremely active in training personnel: 15 graduate students were trained in histological techniques annually, and 8 students or postdoctorals received advanced training in the use of the FARA analyzer, EM, and molecular pathology techniques. It was also used during the summer months in conjunction with an NIH-sponsored Minority Apprenticeship Program for high school students. A variety of provisions have been implemented for future directions since the establishment of MPFC as a result of merger of two laboratories. Dr. Reuhl will retain the overall responsibility for the histology component of the Facility Core functions, while Dr. Lowndes will retain the responsibility of enzymology and immunochemistry activities. The day-to-day operation will be assumed by a technician under the direct supervision of Dr. Reuhl. Upgrading of the Core includes the following: Upgrades for the image analysis/morphology system will be sought to permit greater use of desktop publishing techniques for histological images, immunochemistry, and graphics. Using histopathological expertise present within the EHS Center, a Toxicological Pathology Users Group has been formed to review slides and discuss pathology-related issue. Future upgrading of instruments includes: 1) Software upgrading of the FARA II centrifugal analyzer which is seven years old; and 2) software upgrading of the Presage CV-6 image analysis instrument.