CORE--MOLECULAR PATHOLOGY FACILITY

分子病理学中心（MPFC）成立于1996年 由于合并的细胞酶学设施核心和 组织学实验室。MPFC目前执行各种各样的 评估，包括光学和电子显微镜，图像分析， 免疫细胞化学、原位杂交印迹法（北方、西方 和Southern）和酶分析。MPFC有两个设施： 实验室位于基尔默戈登路的神经毒理学实验室 一个较小的卫星设施位于132、134和136室 位于布希校区的EOHSI大楼 强制性私营公积金的具体目标是：a）向 EHS中心是制备高质量组织学 支持新的和正在进行的研究计划的样本; B）提供 组织病理学评估的专业知识和咨询 样品，并协同协助中心成员在开发和 现代技术在分子病理学中的应用（例如，免疫电子 显微镜，荧光原位杂交）的机制研究， 外源性诱导的损伤; c）提供大规模的迭代酶促 使用FARA II离心酶分析仪分析样品; d） 为调查人员、学生和技术人员提供各种培训 组织形态学的技术和解释;以及e）预测 需要新兴的病理学方法，并建立他们的专业知识， 使用. MPFC的使命和目标是促进 该中心的成员，而不是作为一个纯粹的服务实验室运作。 小额费用由MPFC的业务预算承担。复发性 大型研究的费用是否向用户收取？s赠款.这个核心有 被广泛使用。在过去四年中， 准备并切片用于光学显微镜检查， 用于电子显微镜检查。所有研究核心均使用MPFC。的 最严重的用户是核心I和III，核心II和IV使用较少。 MPFC一直非常积极地培训人员： 学生每年接受组织学技术培训，8名学生或 博士后接受了使用FARA分析仪，EM， 和分子病理学技术。在夏季的几个月里， 结合NIH赞助的少数民族学徒计划， 学校学生。 自2000年以来，为今后的方向实施了各种规定。 由于两个实验室的合并，成立了MPFC。Dr. Bohl 将保留对组织学部分的总体责任， 设施的核心职能，而博士.朗兹将保留的责任， 酶学和免疫化学活性。日常运作将是 由一名技术人员在Dr. Bohl的直接监督下承担。 核心升级包括以下内容：映像升级 将寻求建立一个分析/形态学系统，以便更多地使用台式计算机 用于组织学图像、免疫化学和图形的出版技术。 利用EHS中心内的组织病理学专业知识， 已成立毒理学病理学用户组，以审查载玻片， 讨论病理学相关问题。今后仪器的升级包括： 1)FARA II离心分析仪七年的软件升级 2）Preage CV-6图像分析仪的软件升级。

Molecular Pathology Facility Core (MPFC) was established in 1996 as a result of the merger of the Cellular Enzymology Facility Core and the Histology Laboratory. The MPFC currently performs a wide variety of evaluations, including light- and electron-microscopy, image analysis, immunocytochemistry, in situ hybridization blotting methods (Northern, Western and Southern), and enzyme analysis. The MPFC has two facilities: the central laboratory is located in the Neurotoxicology Laboratory at Gordon Road, Kilmer Campus; and a smaller satellite facility is located in Rooms 132, 134 and 136 of the EOHSI building on Busch Campus. The specific objectives of the MPFC are: a) to make available to members of the EHS Center a facility for the preparation of high-quality histological samples in support of new and ongoing research initiatives; b) to provide expertise and consultation in the pathological assessment of histological samples, and collaboratively to assist Center members in the development and use of contemporary techniques in molecular pathology (e.g., immunoelectron microscopy, fluorescence in situ hybridization) for mechanistic studies of xenobiotic-induced injury; c) to provide large scale, iterative enzymatic analyses of samples using the FARA II centrifugal enzyme analyzer; d) to provide training for investigators, students and technical staff in various technologies and interpretation of tissue morphology; and e) to anticipate the need for emerging pathology methodologies and to establish expertise in their use. The mission and goal of the MPFC is to foster interactive research between members of the Center rather than functioning as a purely service laboratory. Modest costs are absorbed by the operating budget of the MPFC. Recurrent costs for larger studies are charged back to the user?s grants. This Core has been utilized extensively. During the past four years, 14,500 blocks have been prepared and sectioned for light microscopy, and 1,400 samples were embedded for electron microscopy. All Research Cores used the MPFC. The heaviest users were Cores I and III, with Cores II and IV using less heavily. The MPFC has been extremely active in training personnel: 15 graduate students were trained in histological techniques annually, and 8 students or postdoctorals received advanced training in the use of the FARA analyzer, EM, and molecular pathology techniques. It was also used during the summer months in conjunction with an NIH-sponsored Minority Apprenticeship Program for high school students. A variety of provisions have been implemented for future directions since the establishment of MPFC as a result of merger of two laboratories. Dr. Reuhl will retain the overall responsibility for the histology component of the Facility Core functions, while Dr. Lowndes will retain the responsibility of enzymology and immunochemistry activities. The day-to-day operation will be assumed by a technician under the direct supervision of Dr. Reuhl. Upgrading of the Core includes the following: Upgrades for the image analysis/morphology system will be sought to permit greater use of desktop publishing techniques for histological images, immunochemistry, and graphics. Using histopathological expertise present within the EHS Center, a Toxicological Pathology Users Group has been formed to review slides and discuss pathology-related issue. Future upgrading of instruments includes: 1) Software upgrading of the FARA II centrifugal analyzer which is seven years old; and 2) software upgrading of the Presage CV-6 image analysis instrument.