CORE--MOLECULAR GENETICS

描述（由申请人提供）：该核心的总体目的是为中心成员提供服务，科学知识和技术专长，以扩大分子生物学方法在其研究中的应用。该设施核心的具体目标是，1）为研究计划的发展提供支持，这些研究计划可以利用最先进的DNA和RNA分析资源作为服务提供给会员; 2）作为核心分子生物学资源，用于构思和执行新项目和方法，以检查宿主对环境化合物反应的分子机制。 该核心提供资源，以支持该中心环境健康研究中基于DNA的分子方法。该核心为DNA测序、聚合酶链反应、DNA片段分析、DNA微阵列方法、寡核苷酸设计和有限的生物信息学支持提供集中设施。DNA测序实验室提供最新的自动化DNA测序方法，并在很大程度上得到了RWJMS的新泽西癌症研究所的支持，并得到了该中心赠款的额外支持。微阵列服务是自上次审查以来的新增加，新泽西癌症研究所新成立的一个实验室将提供这些服务。合成DNA通过外包机制提供，用于通过与外部商业供应商的合同订购合成寡核苷酸。中心核心强调基本分子生物学技术的教育和指导培训，并主办关于该设施所提供服务的技术研讨会。

Description (provided by applicant): The overall purpose of this core is to provide members of the Center with service, scientific knowledge, and technical expertise to expand the application of molecular biological approaches in their research. The specific objectives of this facility core are, 1) to provide support for the development of research programs that can capitalize on state-of-the-art DNA and RNA analysis resources provided as a service to members; and 2) to serve as a central molecular biology resource for the conception and execution of new projects and approaches for examining the molecular mechanisms of host responses to environmental compounds. This core provides resources to support DNA-based molecular approaches in environmental health research at the Center. The core provides centralized facilities for DNA sequencing, polymerase chain reaction, DNA fragment analysis, DNA microarray methods, oligonucleotide design, and limited bioinformatics support. The DNA sequencing laboratory provides up-to-date methods for automated DNA sequencing and is largely supported by the Cancer Institute of New Jersey at RWJMS with additional support from this Center grant. The microarray service is a new addition since the last review, and a newly established laboratory in the Cancer Institute of New Jersey will provide these services. Synthetic DNA is provided via an outsourcing mechanism for ordering synthetic oligonucleotides through a contract with an outside commercial vendor. The central core emphasizes educational and instructional training in basic molecular biology techniques and sponsors technical seminars on the services provided by the facility.