Vector Core

病媒核心为风湿病核心中心(RDCC)成员提供几项技术 促进特定基因过度表达或减少表达的平台，以帮助研究人员 了解那些与风湿病研究相关的基因的功能和相互作用。 这些平台包括重组非病毒和病毒产品，促进特定基因的转移 基因进入感兴趣的细胞。矢量核心还为RDCC提供智力和技术建议 关于这些系统的最佳使用和生产的研究人员。 尽管分子生物学在风湿病研究中得到了广泛的应用，但许多研究人员 不具备重组载体的工作知识。所需的许多操作技巧 为了最大限度地利用这些基因转移载体，这些载体都是专门化的，没有专家很难获得 援助。此外，这些试剂的制造需要在实验室空间中进行， 已经被特别配置为符合美国国立卫生研究院的生物遏制指南。对以下各项的要求 对这些生物安全指南的遵守阻碍了许多研究人员继续使用这些 有价值的试剂。已经建立了向量核心，以提供具有成本效益的这些资源 有价值的平台，同时最大限度地减少RDCC成员在新实验室上花费时间和金钱的需要 空间和聘请他们自己的矢量专家。 向量核心的具体目标是：1.为开发提供核心实验室， 含研究相关基因重组载体的构建、纯化及鉴定 用于体外和体内基因转移试剂的风湿性疾病。这些系统既包括 非病毒(表达载体)和病毒(重组逆转录病毒、重组腺病毒和重组 AAV)技术。2.与RDCC研究人员密切合作，确保向量核心提供 RDCC成员要求的平台。3.留住合格的员工并监督竞争，以持续 改进向量核心，为RDCC成员提供高质量、高性价比的产品。

The Vector Core provides Rheumatic Diseases Core Center (RDCC) members with several technological platforms to facilitate over-expression or reduced expression of specific genes, to assist researchers in understanding the function and interaction of those genes relevant to the study of rheumatic diseases. These platforms include both recombinant non-viral and viral products that facilitate the transfer of specific genes into cells of interest. The Vector Core also provides intellectual and technical advice to RDCC researchers regarding the optimal use and production of these systems. Despite the wide application of molecular biology to the study of rheumatic diseases, many investigators do not possess a working knowledge of recombinant vectors. Many of the operational techniques required for optimal use of these gene transfer vectors are specialized and difficult to acquire without expert assistance. In addition, the manufacture of these reagents needs to be performed in laboratory space that has been specifically configured to comply with NIH biological containment guidelines. The requirements for compliance with these biosafety guidelines inhibit many investigators from pursuing the use of these valuable reagents. The Vector Core has been established to provide a cost-effective source of these valuable platforms while minimizing RDCC members' need to spend time and money on new laboratory space and hiring their own vector experts. The Specific Aims of the Vector Core are to: 1. Provide a Core laboratory for the development, construction, purification and characterization of recombinant vectors containing genes relevant to the study of rheumatic diseases for use as in vitro and in vivo gene transfer reagents. These systems include both non-viral (expression plasmid) and viral (recombinant retrovirus, recombinant adenovirus, and recombinant AAV) technologies. 2. Collaborate closely with RDCC researchers to ensure the Vector Core provides the platforms RDCC members require. 3. Maintain qualified staff and monitor the competition to continually improve the Vector Core and provide high quality, cost effective products to RDCC members.