Vector Core

矢量核心

• 批准号: 7483084
• 负责人: BLAKE J ROESSLER
• 金额: $ 3.69万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7483084
• 关键词: Adenoviruses; Biological Containment; Cells; Core Facility; Development; Ensure; Facility Construction Funding Category; Gene Transfer; Genes; Guidelines; In Vitro; Knowledge; Laboratories; Molecular Biology; Monitor; Plasmids; Production; Qualifying; Reagent; Recombinants; Research; Research Personnel; Resources; Retroviridae; Rheumatism; Services; Source; System; Techniques; Technology; Time; United States National Institutes of Health; Viral; Work; cost; cost effective; gene transfer vector; improved; in vivo; interdisciplinary collaboration; interest; member; new technology; novel; programs; vector

The Vector Core provides Rheumatic Diseases Core Center (RDCC) members with several technological platforms to facilitate over-expression or reduced expression of specific genes, to assist researchers in understanding the function and interaction of those genes relevant to the study of rheumatic diseases. These platforms include both recombinant non-viral and viral products that facilitate the transfer of specific genes into cells of interest. The Vector Core also provides intellectual and technical advice to RDCC researchers regarding the optimal use and production of these systems. Despite the wide application of molecular biology to the study of rheumatic diseases, many investigators do not possess a working knowledge of recombinant vectors. Many of the operational techniques required for optimal use of these gene transfer vectors are specialized and difficult to acquire without expert assistance. In addition, the manufacture of these reagents needs to be performed in laboratory space that has been specifically configured to comply with NIH biological containment guidelines. The requirements for compliance with these biosafety guidelines inhibit many investigators from pursuing the use of these valuable reagents. The Vector Core has been established to provide a cost-effective source of these valuable platforms while minimizing RDCC members' need to spend time and money on new laboratory space and hiring their own vector experts. The Specific Aims of the Vector Core are to: 1. Provide a Core laboratory for the development, construction, purification and characterization of recombinant vectors containing genes relevant to the study of rheumatic diseases for use as in vitro and in vivo gene transfer reagents. These systems include both non-viral (expression plasmid) and viral (recombinant retrovirus, recombinant adenovirus, and recombinant AAV) technologies. 2. Collaborate closely with RDCC researchers to ensure the Vector Core provides the platforms RDCC members require. 3. Maintain qualified staff and monitor the competition to continually improve the Vector Core and provide high quality, cost effective products to RDCC members.

Vector Core为风湿病核心中心（RDCC）成员提供了几种技术支持。 促进特定基因的过度表达或减少表达的平台，以帮助研究人员 了解与风湿性疾病研究相关的基因的功能和相互作用。 这些平台包括重组非病毒和病毒产品，其促进特异性免疫原性的转移。 将基因导入感兴趣的细胞。Vector Core还为RDCC提供知识和技术咨询 研究人员对这些系统的最佳使用和生产。 尽管分子生物学广泛应用于风湿性疾病的研究， 不具备重组载体的工作知识。需要的许多操作技术 这些基因转移载体的最佳使用是专门的，没有专家的帮助很难获得。 援助.此外，这些试剂的制造需要在实验室空间中进行， 已专门配置为符合NIH生物遏制指南。的要求 遵守这些生物安全指南阻止了许多研究人员继续使用这些生物安全指南。 有价值的试剂矢量核心已经建立，以提供这些成本效益的来源 同时最大限度地减少RDCC成员在新实验室上花费的时间和金钱 空间和雇用他们自己的矢量专家。 矢量核心的具体目标是：1。为开发提供核心实验室， 构建、纯化和表征含有与研究相关的基因的重组载体 作为体外和体内基因转移试剂的用途。这些系统包括两个 非病毒（表达质粒）和病毒（重组逆转录病毒、重组腺病毒和重组 AAV）技术。2.与RDCC研究人员密切合作，确保Vector Core提供 RDCC成员需要的平台。3.保持合格的员工，并监控竞争，以持续 改进Vector Core，为RDCC成员提供高质量、高性价比的产品。