INTEGRATIVE CELL PHENOTYPING AND MORPHOLOGY CORE

综合细胞表型和形态学核心

• 批准号: 7475904
• 负责人: SHERRY L THORNTON
• 金额: $ 11.4万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7475904
• 关键词: Antigens; Apoptosis; Area; Biology; Cell Cycle; Cell Proliferation; Cell Separation; Cell surface; Cells; Data; Development; Disease; Equipment; Flow Cytometry; Gene Expression; Histology; Human Resources; Immunohistochemistry; Immunophenotyping; In Situ Hybridization; Inflammatory; Methodology; Morphology; Pathology; Phenotype; Ploidies; Procedures; Process; Quality Control; Research; Research Personnel; Rheumatism; Standards of Weights and Measures; T-Lymphocyte; Technical Expertise; Techniques; Training; base; cell type; cost; cytokine; release of sequestered calcium ion into cytoplasm; single cell analysis

Several cell types are involved in the inflammatory process in rheumatic diseases. Understanding the biology of these diseases will require dissecting out the contribution of each of these cell subpopulations. The Integrative Cell Phenotyping and Morphology Core will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the research base whose studies require single cell analysis. In this renewal, the former Cell Phenotyping Core has expanded its capabilities to offer expertise in single cell analysis via pathology methodologies. The Core will provide training in the proper use and interpretation of specific immunophenotyping methodologies including flow cytometry, immunohistochemistry, in situ hybridization and histology. Expertise and assistance will be provided by core personnel in the following areas: Cell surface phenotype analysis (flow-based and pathology-based); Morphological cell phenotype analysis (pathology based); Intracellular cytokine analysis (flow-based, and pathology-based); Cell proliferation analysis (flow-based); Cell cycle and DNA index analysis (flow-based); Cell apoptosis analysis (flow-based and pathology-based); Gene expression analysis (flow-based and pathology-based); Calcium flux analysis (flow-based); Cell sorting (flow-based); Antigen-specific T cell analysis (flow-based). In addition, the Core will facilitate the acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the development of new techniques as needed.

几种细胞类型参与风湿性疾病的炎症过程。了解 这些疾病的生物学需要剖析出每个细胞的贡献 亚人群。综合细胞表型和形态学核心将提供技术 以合理的费用向研究基地的研究人员提供专业知识、援助和设备， 研究需要单细胞分析。在这次更新中，以前的细胞表型核心扩展了其 通过病理学方法提供单细胞分析专业知识的能力。核心将提供 正确使用和解释特定免疫表型方法的培训，包括 流式细胞术、免疫组织化学、原位杂交和组织学。专业知识和帮助将 由以下领域的核心人员提供： 细胞表面表型分析（基于流式和病理学）； 形态细胞表型分析（基于病理学）； 细胞内细胞因子分析（基于流式和基于病理学）； 细胞增殖分析（基于流式）； 细胞周期和DNA指数分析（基于流式）； 细胞凋亡分析（基于流式和病理学）； 基因表达分析（基于流式和基于病理学）；钙通量分析（基于流量）； 细胞分选（基于流式）； 抗原特异性 T 细胞分析（基于流式）。 此外，该核心还将促进免疫表型数据的采集、分析和存储， 维护免疫表型分析程序的标准和质量控制，并协助 根据需要开发新技术。