Core 3 - Integrative Cell Phenotyping and Morphology Core

核心 3 - 综合细胞表型和形态学核心

• 批准号: 8381260
• 负责人: SHERRY L THORNTON
• 金额: $ 15.93万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8381260
• 关键词: Address; Antigens; Apoptosis; Area; Arthritis; Autoimmune Diseases; Autoimmune Process; Biological Assay; Biology; Calibration; Cell Cycle; Cell Proliferation; Cell Separation; Cell surface; Cells; Cellular biology; Child; Childhood; Data; Data Analyses; Data Storage and Retrieval; Dendritic Cells; Development; Disease; Equipment; Experimental Designs; Flow Cytometry; Functional disorder; Gene Expression; Goals; Histology; Human Resources; Image; Immune; Immune response; Immune system; Immunohistochemistry; Immunophenotyping; In Situ Hybridization; Inflammatory; Joints; Lasers; Lead; Liquid substance; Lymphocyte; Maintenance; Methodology; Morphology; Pathologic; Pathology; Phenotype; Ploidies; Population; Procedures; Process; Quality Control; Research; Research Personnel; Rheumatism; Sampling; Services; Synovial Fluid; Systemic Lupus Erythematosus; T-Lymphocyte; Technical Expertise; Techniques; Technology; Tissues; Training; base; cell type; cost; cytokine; data management; instrument; macrophage; monocyte; novel therapeutic intervention; release of sequestered calcium ion into cytoplasm; single cell analysis; voltage

Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of spedfic cell subpopulations. The Integrative Cell Phenotyping and Morphology Core (ICPMC) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Research Base of the Cindnnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. The ICPMC will provide training in the proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry, immunohistochemistry, in situ hybridization cind histology. Expertise and assistance will be provided by Core personnel in the following areas: * Cell surface phenotype analysis (flow-based and pathology-based) * Morphological cell phenotype analysis (flow-based and pathology-based) * Intracellular cytokine analysis (flow-based, and pathology-based) * Cell proliferation analysis (flow-based) * Cell cycle and DNA index analysis (flow-based) * Cell apoptosis analysis (flow-based and pathology-based) * Gene expression analysis (flow-based and pathology-based) * Calcium flux analysis (flow-based) * Cell sorting (flow-based) * Antigen-specific T cell analysis (flow-based) In addition, the ICPMC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the application and development of new techruques.

几种细胞类型参与风湿性疾病的炎症过程。了解这些疾病的病理生物学通常需要解剖出特定细胞亚群的贡献。综合细胞表型和形态学核心（ICPMC）将以合理的成本为Cindnnati风湿病核心中心（CRDCC）研究基地的研究人员提供技术专业知识，援助和设备，其研究需要单细胞分析。 委员会将提供关于正确使用和解释若干 免疫分型方法，包括流式细胞术、免疫组织化学、原位杂交和组织学。核心人员将在以下领域提供专门知识和协助： * 细胞表面表型分析（基于流动和基于病理学） * 形态学细胞表型分析（基于流动和基于病理） * 细胞内细胞因子分析（基于流量和基于病理学） * 细胞增殖分析（基于流动） * 细胞周期和DNA指数分析（基于流式细胞仪） * 细胞凋亡分析（基于流式细胞术和基于病理学） * 基因表达分析（基于流程和基于病理学） * 钙通量分析（基于流量） * 细胞分选（基于流动） * 抗原特异性T细胞分析（基于流式细胞仪） 此外，ICPMC将促进免疫分型数据的获取、分析和储存，维护免疫分型程序的标准和质量控制，并协助新技术的应用和开发。