Integrative Cell Phenotyping Core

综合细胞表型分析核心

• 批准号: 10704367
• 负责人: SHERRY L THORNTON
• 金额: $ 16.05万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10704367
• 关键词: Address; Apoptosis; Area; Arthritis; Autoimmune Diseases; Autoimmune Process; Bioinformatics; Biological; Biology; Calibration; Cell Cycle; Cell Proliferation; Cell Separation; Cell surface; Cells; Cellular biology; Child; Childhood; Chronic Childhood Arthritis; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Data; Data Analyses; Data Storage and Retrieval; Dendritic Cells; Detection; Development; Disease; Equipment; Experimental Designs; Flow Cytometry; Functional disorder; Gene Expression; Gene Expression Profiling; Genetic Transcription; Genomics; Goals; Health Services Accessibility; Human Resources; Image Cytometry; Immune; Immune response; Immune system; Immunologics; Immunophenotyping; Individual; Inflammation Mediators; Inflammatory; Joints; Juvenile Dermatomyositis; Lasers; Lymphocyte; Macrophage; Maintenance; Methodology; Modeling; Pathologic; Pathway interactions; Phenotype; Ploidies; Population; Procedures; Process; Protein Secretion; Proteins; Proteomics; Quality Control; RNA; Reporter; Reproducibility; Research; Research Personnel; Resources; Rheumatism; Rheumatoid Arthritis; Rheumatology; Sampling; Services; Signal Transduction; Sorting; Stains; Synovial Fluid; Systemic Lupus Erythematosus; Technical Expertise; Techniques; Technology; Tissues; Training; antigen-specific T cells; autoimmune rheumatologic disease; autoinflammatory; autoinflammatory diseases; cell type; cost; data cleaning; data management; design; functional genomics; genome editing; high dimensionality; instrument; monocyte; novel; novel therapeutic intervention; pediatric rheumatic diseases; release of sequestered calcium ion into cytoplasm; single cell analysis; single cell proteins; transcriptome sequencing; transcriptomics; voltage

Abstract – Integrative Cell Phenotyping Core (ICPC) Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of specific cell subpopulations. The ICPC (Integrative Cell Phenotyping Core) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Cincinnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. ICPC provides training in proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry, multiplex analyte analysis and single cell genomic, proteomic and spatial transcriptomic analysis. Expertise and assistance will be provided by Core personnel in the following areas: • High-parameter immunophenotyping (cell surface and intracellular) • Single cell gene expression analysis • Cell sorting • Gene expression reporter analysis • Cell proliferation analysis • Cell cycle and DNA content analysis • Apoptosis analysis • Intracellular calcium mobilization analysis • Tetramer staining of antigen-specific T cells • RNA detection by flow cytometry • Cell signalling analysis • CRISPR genome-edited cell identification and sorting • Imaging cytometry • Multiplex protein/analyte detection • Single cell protein secretion • Spatial transcriptomics In addition, the ICPC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping, single cell gene expression, and spatial transcriptomics procedures, and will assist in the application and development of new techniques.

摘要-整合细胞表型核心（ICPC） 几种细胞类型参与风湿性疾病的炎症过程。了解 这些疾病的病理生物学通常需要解剖出特定细胞亚群的贡献。的 ICPC（整合细胞表型核心）将提供技术专长，援助和设备， 辛辛那提风湿病核心中心（CRDCC）研究者的合理费用， 需要单细胞分析。国际预防犯罪中心提供关于正确使用和解释一些 免疫分型方法，包括流式细胞术、多重分析物分析和单细胞 基因组学、蛋白质组学和空间转录组学分析。核心小组将提供专门知识和协助。 以下领域的人员： ·高参数免疫分型（细胞表面和细胞内） ·单细胞基因表达分析 ·细胞分选 ·基因表达报告分析 ·细胞增殖分析 ·细胞周期和DNA含量分析 ·细胞凋亡分析 ·细胞内钙动员分析 ·抗原特异性T细胞的四聚体染色 ·通过流式细胞术进行RNA检测 ·细胞信号传导分析 · CRISPR基因组编辑的细胞鉴定和分选 ·成像细胞仪 ·多重蛋白质/分析物检测 ·单细胞蛋白质分泌 ·空间转录组学 此外，ICPC将促进免疫表型数据的采集、分析和存储， 免疫表型、单细胞基因表达和空间转录组学的标准和质量控制 并将协助应用和开发新技术。