Integrative Cell Phenotyping Core

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Abstract – Integrative Cell Phenotyping Core (ICPC) Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of specific cell subpopulations. The ICPC (Integrative Cell Phenotyping Core) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Cincinnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. ICPC provides training in proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry, multiplex analyte analysis and single cell genomic, proteomic and spatial transcriptomic analysis. Expertise and assistance will be provided by Core personnel in the following areas: • High-parameter immunophenotyping (cell surface and intracellular) • Single cell gene expression analysis • Cell sorting • Gene expression reporter analysis • Cell proliferation analysis • Cell cycle and DNA content analysis • Apoptosis analysis • Intracellular calcium mobilization analysis • Tetramer staining of antigen-specific T cells • RNA detection by flow cytometry • Cell signalling analysis • CRISPR genome-edited cell identification and sorting • Imaging cytometry • Multiplex protein/analyte detection • Single cell protein secretion • Spatial transcriptomics In addition, the ICPC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping, single cell gene expression, and spatial transcriptomics procedures, and will assist in the application and development of new techniques.