Single Cell Phenotyping Core

几种细胞类型参与风湿性疾病的炎症过程。了解 这些疾病的病理生物学通常需要解剖出特定细胞亚群的贡献。 SCPC（单细胞表型核心）将提供技术专长，援助和设备， 辛辛那提风湿病核心中心研究基地研究者的合理费用 （CRDCC），其研究需要单细胞分析。SCPC将提供正确使用的培训， 解释了一些免疫表型分析方法，包括流式细胞术和单细胞 基因表达。核心人员将在以下领域提供专门知识和协助： ·细胞分选 ·细胞表面表型分析 ·细胞内细胞因子检测 ·基因表达报告分析 ·细胞增殖分析 ·细胞周期和DNA含量分析 ·细胞凋亡分析 ·细胞内钙动员分析 ·抗原特异性T细胞的四聚体染色 ·通过流式细胞术进行RNA检测 ·细胞信号传导分析 · CRISPR基因组编辑的细胞鉴定和分选 ·成像细胞仪 ·单细胞操作和基因表达分析 此外，SCPC将促进免疫表型数据的采集、分析和存储， 免疫分型程序的标准和质量控制，并协助应用和 开发新技术。

Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of specific cell subpopulations. The SCPC (Single Cell Phenotyping Core) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Research Base of the Cincinnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. The SCPC will provide training in the proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry and single cell gene expression. Expertise and assistance will be provided by Core personnel in the following areas: · Cell sorting · Cell surface phenotyping · Intracellular cytokine detection · Gene expression reporter analysis · Cell proliferation analysis · Cell cycle and DNA content analysis · Apoptosis analysis · Intracellular calcium mobilization analysis · Tetramer staining of antigen-specific T cells · RNA detection by flow cytometry · Cell signalling analysis · CRISPR genome-edited cell identification and sorting · Imaging cytometry · Single cell manipulation and gene expression analysis In addition, the SCPC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the application and development of new techniques.