Low-Cost Digital Imaging System for Evaluating Thrombosis

用于评估血栓形成的低成本数字成像系统

基本信息

  • 批准号:
    7465416
  • 负责人:
  • 金额:
    $ 7.42万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-09-01 至 2009-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Clinical thrombosis has a series and wide-ranging impact on the health of millions of patients, as occurs with heart attacks, strokes, venous thrombo-embolism and disseminated intravascular coagulation. The experimental study of thrombosis requires a consideration of many converging elements within a single physical locale - an intravascular thrombus. The interplay of coagulation factors and platelet activation/accumulation is incompletely understood, as well as the relative involvement of each element in arterial or venous or microvascular thrombosis - distinct entities with different mechanisms of thrombus development and resolution. To overcome the high cost and labor-intensive aspects of recently developed systems for thrombosis study, and to adapt such a system to large-vessel (both artery and vein) thrombosis investigations, the following proposed study will develop a low-cost imaging system for continuous quantitative evaluation of a developing thrombus. Murine large arteries and veins (up to 1 mm in diameter) will be imaged with a low-light digital camera with video development capabilities. Thrombus targets will be labeled with fluorophores (attached to systemically circulating platelets or fibrinogen, or antibodies to coagulation cascade enzymes) to permit monitoring of incorporation into a standardly induced thrombus (using thrombosis models developed and published by the PI). Low-cost excitation light sources (e.g., LEDs or laser pointers) will be matched to the excitation wavelength of each fluorophore, using band-pass filters to permit image capture of emission wavelengths for each fluorophore. The system will undergo rigorous ex vivo testing, followed by in vivo analyses, directly comparing outcomes with established methods for thrombus quantitation. The findings will demonstrate the feasibility of developing such a system and show its applicability to the investigation of experimental thrombosis. This imaging methodology may have a capacity for future adaptation to clinical thrombosis imaging.
描述(由申请人提供):临床血栓形成对数百万患者的健康产生一系列广泛的影响,如心脏病发作、中风、静脉血栓栓塞和弥散性血管内凝血。血栓形成的实验研究需要考虑单个物理部位(血管内血栓)内的许多汇聚元素。凝血因子和血小板活化/积聚的相互作用以及动脉或静脉或微血管血栓形成中每种元素的相对参与尚不完全清楚——不同的实体具有不同的血栓形成和消退机制。为了克服最近开发的血栓研究系统的高成本和劳动密集型问题,并使该系统适应大血管(动脉和静脉)血栓形成研究,以下拟议研究将开发一种低成本成像系统,用于对正在形成的血栓进行连续定量评估。小鼠大动脉和静脉(直径达 1 毫米)将使用具有视频开发功能的低光数码相机进行成像。血栓目标将用荧光团(附着于全身循环的血小板或纤维蛋白原,或凝血级联酶的抗体)进行标记,以允许监测并入标准诱导的血栓(使用 PI 开发和发布的血栓形成模型)。低成本激发光源(例如 LED 或激光笔)将与每个荧光团的激发波长相匹配,使用带通滤光片来捕获每个荧光团发射波长的图像。该系统将经过严格的离体测试,然后进行体内分析,直接将结果与现有的血栓定量方法进行比较。研究结果将证明开发这种系统的可行性,并展示其对实验性血栓形成研究的适用性。这种成像方法可能有能力在未来适应临床血栓形成成像。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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BRIAN CROCKETT COOLEY其他文献

BRIAN CROCKETT COOLEY的其他文献

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{{ truncateString('BRIAN CROCKETT COOLEY', 18)}}的其他基金

Effect of Osteoporosis on Spine Fusion
骨质疏松症对脊柱融合的影响
  • 批准号:
    7821313
  • 财政年份:
    2008
  • 资助金额:
    $ 7.42万
  • 项目类别:
Effect of Osteoporosis on Spine Fusion
骨质疏松症对脊柱融合的影响
  • 批准号:
    7531155
  • 财政年份:
    2008
  • 资助金额:
    $ 7.42万
  • 项目类别:
Effect of Osteoporosis on Spine Fusion
骨质疏松症对脊柱融合的影响
  • 批准号:
    7673616
  • 财政年份:
    2008
  • 资助金额:
    $ 7.42万
  • 项目类别:
Low-Cost Digital Imaging System for Evaluating Thrombosis
用于评估血栓形成的低成本数字成像系统
  • 批准号:
    7293875
  • 财政年份:
    2007
  • 资助金额:
    $ 7.42万
  • 项目类别:
Effect of Age on Vein Graft Neointimal Formation
年龄对静脉移植新内膜形成的影响
  • 批准号:
    6684679
  • 财政年份:
    2003
  • 资助金额:
    $ 7.42万
  • 项目类别:

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