International Collaboration in Chemistry: Mechanism of Operation of the BLUF Domain - Blue Light Sensitive Biosensors

国际化学合作：BLUF 结构域的运作机制 - 蓝光敏感生物传感器

• 批准号: EP/G002916/1
• 负责人: Stephen Meech
• 金额: $ 36.38万
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FG002916%2F1
• 关键词: International; Collaboration; Chemistry; Mechanism; Operation

Plants and animals respond to light - the growth of a plant toward sunlight is an example (our own 'body clock' may be another). In this project we investigate the mode of operation of one recently discovered blue light sensitive protein, which controls the expression of bacterial photosynthesis. This study requires complementary skills in protein chemistry, spectroscopy and dynamics, which are a particular feature of the research team. What is known about the light sensing mechanism is that blue light absorbed by a flavin produces a structural change in the protein. What is not known is how and how fast. Our first objective will be to use the structural and dynamic information available from ultrafast electronic and vibrational spectroscopy to address these questions in the native protein. To do this we will use a traditional tool of spectroscopy - but one which is difficult to apply to proteins - isotope substitution. Having characterised the mechanism we will then seek to modify and enhance it through chemical manipulation of the protein structure. This research addresses the fundamental mechanism of operation of the light sensitive protein, but our long term aim is to regulate and enhance the blue light response to develop photoactive proteins, which might, for example, be used for light activated drug release.

植物和动物对光有反应--植物朝着阳光生长就是一个例子（我们自己的“生物钟”可能是另一个例子）。在这个项目中，我们研究了一个最近发现的蓝光敏感蛋白的操作模式，它控制细菌光合作用的表达。这项研究需要蛋白质化学，光谱学和动力学方面的互补技能，这是研究团队的一个特点。已知的光感应机制是，蓝光被黄素吸收后，会引起蛋白质的结构变化。我们不知道的是如何以及有多快。我们的第一个目标是利用超快电子和振动光谱学提供的结构和动力学信息来解决天然蛋白质中的这些问题。为了做到这一点，我们将使用一种传统的光谱学工具--同位素替代，但这种工具很难应用于蛋白质。在描述了该机制之后，我们将通过对蛋白质结构的化学操作来修改和增强它。这项研究解决了光敏蛋白的基本运作机制，但我们的长期目标是调节和增强蓝光反应，以开发光活性蛋白，例如，可用于光激活药物释放。

项目成果

BLUF domain function does not require a metastable radical intermediate state.

BLUF结构域功能不需要亚稳组织中间状态。

• DOI: 10.1021/ja4121082
• 发表时间: 2014-03-26
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Lukacs A;Brust R;Haigney A;Laptenok SP;Addison K;Gil A;Towrie M;Greetham GM;Tonge PJ;Meech SR
• 通讯作者: Meech SR

Proteins in action: femtosecond to millisecond structural dynamics of a photoactive flavoprotein.

• DOI: 10.1021/ja407265p
• 发表时间: 2013-10-30
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Brust R;Lukacs A;Haigney A;Addison K;Gil A;Towrie M;Clark IP;Greetham GM;Tonge PJ;Meech SR
• 通讯作者: Meech SR

Ultrafast proton transfer in the green fluorescent protein: Analysing the instantaneous emission at product state wavelengths

绿色荧光蛋白中的超快质子转移：分析产物状态波长下的瞬时发射

• DOI: 10.1016/j.jphotochem.2011.12.016
• 发表时间: 2012
• 期刊: Chemistry
• 作者: Kondo M