Nanoparticle-based Mannetic Microluidic Enrichment System (MMES)
基于纳米颗粒的磁力微流控富集系统 (MMES)
基本信息
- 批准号:7483314
- 负责人:
- 金额:$ 17.33万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-03-04 至 2009-02-28
- 项目状态:已结题
- 来源:
- 关键词:Abnormal CellAffinityAntibodiesBindingBiological MarkersBladderBlood specimenBody FluidsBreast Cancer CellCancer DetectionCancer cell lineCell LineCell SeparationCell modelCell surfaceCellsCervix UteriChemistryColonCongenital AbnormalityCorrelation StudiesDevelopmentDiagnosticDiagnostic Neoplasm StagingEpidermal Growth Factor ReceptorEpigenetic ProcessEpithelial CellsEsophagusFluorescence-Activated Cell SortingG-substrateGene ProteinsGenerationsGenomicsGenus ColaHumanImmunomagnetic SeparationIn VitroLeadLesionLigandsLungMagnetismMalignant NeoplasmsMethodsMicrofluidicsMicrospheresNanotechnologyNormal CellNumbersOceansOperative Surgical ProceduresOral cavityParticle SizePeptidesPhasePremalignantProceduresPropertyProstateProtein OverexpressionProteomicsPublic HealthRangeRateRecoveryResearchScreening for cancerSmall Business Funding MechanismsSmall Business Innovation Research GrantSorting - Cell MovementSputumStagingStomachSurfaceSystemTechnologyUnited States National Institutes of HealthUrineWorkbasecancer cellcostdesigneffusionepigenomicserbB-2 Receptorexperiencein vivoiron oxidemagnetic beadsnanoparticleneoplastic cellprototypereceptorsizetumor
项目摘要
DESCRIPTION (provided by applicant): Enriched abnormal cells will enable genomic, proteomic, and epigenomic analyses to detect cancer-specific alterations in expressed genes, protein/peptide profiles, and epigenetic markers. The ability to carry out such enrichment in high efficiency in a routine way using body fluids may lead to improvements in cancer detection at early stage. The current enrichment methods rely on fluorescence-activated cell sorting (FACS) and immunomagnetic separation (IMS). FACS is an emerging diagnostic field which offers the isolation of cell subsets with high purity however, its sorting rate (104 cells/s) is slow and its apparatus is large and expensive. IMS is simple and low cost method to separate targeting cells however, the batch operation limits its throughput and its enrichment factor is low. This NIH SBIR Phase I proposal is to develop a simple, high throughput and high enrichment factor system for the enrichment of rare cancer cells. In phase I, we will demonstrate the feasibility of using size tunable iron oxide magnetic nanoparticles (MNPs) for the enrichment of cancer cells with high enrichment factor. In phase II, we will optimize this system by focusing on a specific cancer cell in body fluid. The final deliverables of this project will include columnless kit, column kit, and the magnetic microfluidic concentrator for different level of enrichment. PUBLIC HEALTH RELEVANCE: More than 80 percent of human tumors originate from epithelial cells, often at a mucosal surface, and are clonal in origin (e.g., colon, lung, prostate, oral cavity, esophagus, stomach, uterine cervix, bladder). Their development often becomes apparent when tumor cells exfoliate spontaneously into sputum, urine, or even into various effusions. The molecular and genetic abnormalities within these exfoliated cells could be used to detect and identify precancerous lesions or very early stage cancer if highly sensitive technologies were clinically available to identify the few abnormal cells among millions of normal cells. Successful development of this project will provide a high throughput and high enrichment factor system for the enrichment of rare cancer cells and cell fragments in body fluids for early cancer detection.
描述(由申请人提供):富集的异常细胞将使基因组、蛋白质组和表观基因组分析能够检测表达基因、蛋白质/肽谱和表观遗传标记中的癌症特异性改变。使用体液以常规方式高效地进行这种富集的能力可能导致早期癌症检测的改进。目前的富集方法依赖于荧光激活细胞分选(FACS)和免疫磁性分离(IMS)。流式细胞术是一个新兴的诊断领域,它提供了高纯度的细胞亚群的分离,然而,它的分选速度(104个细胞/秒)是缓慢的,它的设备是大的和昂贵的。IMS是一种简单、低成本的分离靶细胞的方法,但批量操作限制了其通量,且富集因子较低。该NIH SBIR I期提案旨在开发一种简单、高通量和高富集因子的系统,用于富集罕见癌细胞。在第一阶段,我们将证明使用尺寸可调的氧化铁磁性纳米颗粒(MNP)富集具有高富集因子的癌细胞的可行性。在第二阶段,我们将通过关注体液中的特定癌细胞来优化该系统。本项目的最终成果将包括无柱试剂盒、柱试剂盒和用于不同富集水平的磁性微流控浓缩器。 公共卫生关系:超过80%的人类肿瘤起源于上皮细胞,通常在粘膜表面,并且是克隆起源(例如,结肠、肺、前列腺、口腔、食道、胃、子宫颈、膀胱)。当肿瘤细胞自发脱落到痰、尿甚至各种渗出液中时,它们的发展往往变得明显。这些脱落细胞内的分子和遗传异常可用于检测和识别癌前病变或非常早期的癌症,如果临床上可获得高灵敏度的技术来识别数百万正常细胞中的少数异常细胞。该项目的成功开发将为富集体液中的罕见癌细胞和细胞碎片提供高通量和高富集因子系统,用于早期癌症检测。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Yongqiang Andrew Wang其他文献
Yongqiang Andrew Wang的其他文献
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{{ truncateString('Yongqiang Andrew Wang', 18)}}的其他基金
Supplementary for Assessment of Microvessel Density and Tumor Proximity for Prognosis of Cancer
评估微血管密度和肿瘤邻近性以评估癌症预后的补充
- 批准号:
9194361 - 财政年份:2014
- 资助金额:
$ 17.33万 - 项目类别:
Assessment of microvessel density and tumor proximity for prognosis of cancer
评估微血管密度和肿瘤邻近性对癌症预后的影响
- 批准号:
8647495 - 财政年份:2014
- 资助金额:
$ 17.33万 - 项目类别:
Magnetic Nanoparticles and Quantum Dots for Diagnosis of Early Stage Ovarian Canc
磁性纳米颗粒和量子点用于诊断早期卵巢癌
- 批准号:
8110007 - 财政年份:2010
- 资助金额:
$ 17.33万 - 项目类别:
Application of QDs-based nanotyping technology in the evaluation of chemotherapy
基于量子点的纳米分型技术在化疗评价中的应用
- 批准号:
7746654 - 财政年份:2009
- 资助金额:
$ 17.33万 - 项目类别:
Iron oxide nanoparticle probes for target specific MR molecular imaging
用于目标特异性 MR 分子成像的氧化铁纳米颗粒探针
- 批准号:
7611718 - 财政年份:2009
- 资助金额:
$ 17.33万 - 项目类别:
Development of Cadmium-free and Multicolor Quantum Dots as Lifetime Imaging Probe
开发无镉多色量子点作为终身成像探针
- 批准号:
7483313 - 财政年份:2008
- 资助金额:
$ 17.33万 - 项目类别:
Nanoparticle Based Magnetic Microfluidic concentrator (MMC)
基于纳米颗粒的磁微流控浓缩器 (MMC)
- 批准号:
7348254 - 财政年份:2008
- 资助金额:
$ 17.33万 - 项目类别:
Multifunctional nanoparticles for multiplex detection of breast cancer biomarkers
用于乳腺癌生物标志物多重检测的多功能纳米颗粒
- 批准号:
7939574 - 财政年份:2006
- 资助金额:
$ 17.33万 - 项目类别:
Adaptor Protein-Quantum Dot Conjugates as Biological Labels
接头蛋白-量子点缀合物作为生物标记
- 批准号:
7110878 - 财政年份:2006
- 资助金额:
$ 17.33万 - 项目类别:
Multifunctional nanoparticles for multiplex detection of breast cancer biomarkers
用于乳腺癌生物标志物多重检测的多功能纳米颗粒
- 批准号:
7612791 - 财政年份:2006
- 资助金额:
$ 17.33万 - 项目类别:
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