Development of Cadmium-free and Multicolor Quantum Dots as Lifetime Imaging Probe
开发无镉多色量子点作为终身成像探针
基本信息
- 批准号:7483313
- 负责人:
- 金额:$ 17.46万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-03-01 至 2009-02-28
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAntibodiesBindingBiocompatibleBiologicalBiological MarkersBuffersC-terminalCCL4 geneCadmiumCell LineCellsClassColorCompatibleCountCouplingDevelopmentElementsEngineeringEnvironmentEvaluationFluorescenceGenerationsGoalsHistidineHumanImageImaging TechniquesIn VitroIonsLabelLigandsLiliumLinkLocationMeasurementMethodsMonitorN-terminalNickelNude MiceOceansParaffin EmbeddingParticle SizePeptidesPhasePhotonsPolymersPreparationPropertyProteinsQuantum DotsRangeReagentSemiconductorsSmall Business Funding MechanismsSmall Business Innovation Research GrantSolutionsSpecificityStandards of Weights and MeasuresSurfaceTestingThickThree-Dimensional ImageThree-Dimensional ImagingTimeTissuesTumor Cell LineTumor TissueUnited States National Institutes of HealthUniversitiesWaterWidthYangabsorptionactivating transcription factorbasebiocompatible polymerchemical stabilitycopolymercrosslinkdesirefluorophoreimaging probeimprovedin vivointerestnanocrystalneoplastic cellnovel strategiesquantumresearch studysizetime usetumor xenograft
项目摘要
DESCRIPTION (provided by applicant): Since semiconductor nanocrystals [quantum dots (QDs)] have been adopted as a new class of fluorescent labels, many studies have indicated a broad range of potential applications using QDs as new probes in vivo and in vitro imaging. Because of QDs long lifetimes (10s of ns) compared to organic fluorophores (1-5 ns), QDs can be used as new fluorescence lifetime imaging probes to reduce cells and tissue's autofluorescence background (2-4 ns). This NIH SBIR project will focus on developing a new generation of cadmium-free, water-soluble multicolor QDs for lifetime imaging to eliminate the autofluorescence for in vitro QD-imaging of biomarkers in tumor cells and tissue sections. A key feature of this new approach is to tune QD emission color by doping the QD core with a third element rather than changing the particle sizes. This kind of new type doped QDs should bring wide interest in the applications of fluorescence labeling due to long lifetime emissions from doped ion itself and possible high emission efficiency by QDs as donors and doped ions as accepters. The doped core/shell QDs would be converted to water soluble and biocompatible for the potential biomedical applications in the field of real-time imaging.
描述(由申请人提供):由于半导体纳米晶体[量子点(QD)]已被用作一类新的荧光标记,许多研究表明,使用QD作为体内和体外成像的新探针具有广泛的潜在应用。由于与有机荧光团(1-5 ns)相比,QD的寿命长(10 ns),QD可以用作新的荧光寿命成像探针,以减少细胞和组织的自发荧光背景(2-4 ns)。该NIH SBIR项目将专注于开发新一代无镉、水溶性的生物标记物量子点,用于终身成像,以消除肿瘤细胞和组织切片中生物标记物体外量子点成像的自发荧光。这种新方法的一个关键特征是通过用第三种元素掺杂QD核心来调节QD发射颜色,而不是改变颗粒尺寸。这种新型的掺杂量子点由于掺杂离子本身的长寿命发射以及量子点作为施主和掺杂离子作为受主可能的高发射效率,在荧光标记应用中将引起广泛的兴趣。掺杂的核/壳量子点将被转化为水溶性和生物相容性的潜在的生物医学应用领域的实时成像。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Yongqiang Andrew Wang其他文献
Yongqiang Andrew Wang的其他文献
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{{ truncateString('Yongqiang Andrew Wang', 18)}}的其他基金
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Assessment of microvessel density and tumor proximity for prognosis of cancer
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7746654 - 财政年份:2009
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Iron oxide nanoparticle probes for target specific MR molecular imaging
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$ 17.46万 - 项目类别:
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Multifunctional nanoparticles for multiplex detection of breast cancer biomarkers
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- 批准号:
7612791 - 财政年份:2006
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