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Magnetic Nanoparticles and Quantum Dots for Diagnosis of Early Stage Ovarian Canc

磁性纳米颗粒和量子点用于诊断早期卵巢癌

基本信息

批准号：
8110007
负责人：
Yongqiang Andrew Wang
金额：
$ 14.12万
依托单位：
OCEAN NANOTECH, LLC
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-12 至 2012-12-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8110007
关键词：
Achievement American Antibodies Binding Biological Biological Assay Biological Markers Biological Process Biopsy Biosensor Buffers Build-it CA-125 Antigen Caliber Cancer Patient Cells Clinical Clinics and Hospitals Color Comparative Study Cytochromes Decision Making Detection Development Devices Diagnosis Diagnostic Diagnostic Neoplasm Staging Diagnostic Procedure Disease Early Diagnosis Early treatment Electrodes Electron Transport Engineering Epidemiology Epididymis Equipment Exhibits Fluorescence Fluorescent Antibody Technique Foundations Fred Hutchinson Cancer Research Center G-substrate Generations Goals Gold High Risk Woman Hour Human Hybrids Image Immobilization Immunoassay Immunomagnetic Separation Individual Label Lead Letters Lighting Liquid substance Magnetism Malignant Neoplasms Malignant neoplasm of ovary Manuals Marketing Mechanics Medical Membrane Methods Microfluidics Microspheres Modeling Monitor Nanotechnology Oceans Optics Ovarian Patients Pharmaceutical Preparations Phase Precipitation Preparation Printing Property Protein Array Proteins Pump Quantum Dots Reading Reagent Reliability of Results Reporter Reporting Research Rest Sampling Scheme Screening for Ovarian Cancer Screening for cancer Serum Signal Transduction Solutions Source Staging Surface Survival Rate Symptoms Syringes System Techniques Technology Therapeutic Therapeutic Agents Time Ultrasonography United States National Institutes of Health Universities Vaccines WFDC2 gene Washington West Virginia Woman Work austin base cancer diagnosis cancer type clinical Diagnosis cost density design drug development drug efficacy efficacy testing gel electrophoresis high throughput analysis high throughput screening hospital laboratories improved innovation instrument iron oxide magnetic beads mesothelin micro-total analysis system miniaturize nano nanocrystal nanofabrication nanoparticle nanoscale novel preclinical study programs prototype public health relevance quantum rapid detection response seal sensor success tool ultraviolet user-friendly vaccine development

项目摘要

DESCRIPTION (provided by applicant): Ocean NanoTech proposes to develop a portable, sensitive, high throughput, low cost, and user friendly system for the early stage diagnosis of ovarian cancer using magnetic nanoparticles (MNPs), quantum dots (QDs), and a nanoelectrode array (NEA) all integrated in a disposable lab-on-a-chip. The proposed system will consists of a fully automated instrument that requires a disposable lab-on-a-chip device that uses very sensitive high quantum yield quantum dots and nanoelectrode array for signal generation in the diagnosis of stage I levels of ovarian cancer biomarkers at concentrations potentially d 1 ng/mL in a d 60 min assay. It will be fully automated allowing high throughput analysis, high sensitivity with > 95% reliability, and minimizing false positives and false negatives. Unlike existing diagnostic methods of ovarian cancer which are either invasive or detect biomarkers too late in the progress of the disease leading to very low survival rate, the proposed system is anticipated to detect early stages of the disease which is the most promising approach to the long term survival of ovarian cancer patients. The proposed system is anticipated to diagnose early stage ovarian cancer that can be used for the following very important purposes: 1) regular monitoring of high risk women; 2) diagnose early stage of the disease at the onset of vague clinical symptoms; 3) monitor changes in the ovarian cancer biomarker levels during medication; and 4) detection system during the development and efficacy testing of drugs, vaccine, or other therapeutic molecules. PUBLIC HEALTH RELEVANCE: Our goal is to develop a portable, sensitive, high throughput, low cost, and user friendly system for the early stage diagnosis of ovarian cancer that uses a disposable lab-on-a-chip that integrates the advantages of magnetic nanoparticles (MNPs), quantum dots (QDs), and nanoelectrode array (NEA) for fluorescence as well as nanoelectrochemical detection. We will develop the best combination of MNPs, QDs, and NEA for isolation and detection of multiple ovarian cancer biomarkers for early diagnosis and possible early treatment of approximately 20,000 American women who are diagnosed with ovarian cancer and of whom 15,000 die each year.

描述（由申请人提供）：Ocean NanoTech提出开发一种便携式，灵敏，高通量，低成本和用户友好的系统，用于卵巢癌的早期诊断，使用磁性纳米颗粒（MNP），量子点（QD）和纳米电极阵列（NEA），所有这些都集成在一次性实验室芯片中。所提出的系统将由一个全自动仪器组成，该仪器需要一次性芯片实验室设备，该设备使用非常敏感的高量子产率量子点和纳米电极阵列，用于在60分钟测定中以潜在的d 1 ng/mL浓度诊断卵巢癌生物标志物的I期水平。它将完全自动化，允许高通量分析，高灵敏度，> 95%的可靠性，并最大限度地减少假阳性和假阴性。与现有的卵巢癌诊断方法不同，现有的卵巢癌诊断方法要么是侵入性的，要么在疾病进展中太晚检测生物标志物，导致生存率非常低，预计所提出的系统可以检测疾病的早期阶段，这是卵巢癌患者长期生存的最有希望的方法。所提出的系统预期用于诊断早期卵巢癌，其可用于以下非常重要的目的：1）定期监测高危女性; 2）在模糊临床症状发作时诊断疾病的早期阶段; 3）监测药物治疗期间卵巢癌生物标志物水平的变化;以及4）在药物、疫苗或其它治疗分子的开发和功效测试期间的检测系统。公共卫生相关性：我们的目标是开发一种便携式，灵敏，高通量，低成本和用户友好的系统，用于卵巢癌的早期诊断，该系统使用一次性芯片实验室，该芯片集成了磁性纳米颗粒（MNP），量子点（QD）和纳米电极阵列（NEA）的优点，用于荧光和纳米电化学检测。我们将开发MNP，QD和NEA的最佳组合，用于分离和检测多种卵巢癌生物标志物，用于早期诊断和可能的早期治疗约20，000名被诊断患有卵巢癌的美国妇女，其中每年有15，000人死亡。