Detection of Environmental Agents Using a Novel Aerosol Sampler Coupled to a Micr

使用与显微镜耦合的新型气溶胶采样器检测环境因子

• 批准号: 7500072
• 负责人: Peter C. Ariessohn
• 金额: $ 26.86万
• 依托单位: ENERTECHNIX, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-24 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7500072
• 关键词: Aerosols; Air; Area; Biological; Biological Assay; Blood capillaries; Cell Wall; Chemical Agents; Chemicals; Chemistry; Collection; Coupled; Data; Detection; Diagnostic Procedure; Ecology; Endotoxins; Engineering; Environment; Equipment; Event; Fluorescence; Fluorescence Microscopy; Funding; Government; Gram-Negative Bacteria; Grant; Health; Hour; Immunoassay; Inflammatory; Link; Lipopolysaccharides; Liquid substance; Localized; Logic; Measurable; Measurement; Membrane; Methods; Microbiology; Microfluidics; Modeling; Monitor; Nucleic Acids; Performance; Phase; Polymerase Chain Reaction; Positioning Attribute; Preparation; Process; Production; Range; Reagent; Research; Sampling; Security; Series; Standards of Weights and Measures; System; Technology; Testing; Time; Toxicology; Uncertainty; Universities; Washington; air sampling; base; capillary; design; environmental agent; exposed human population; improved; instrument; laboratory facility; lens; light weight; miniaturize; monitoring device; novel; particle; pollutant; prototype; response; tool

DESCRIPTION (provided by applicant) The proposed project will develop and demonstrate a personal exposure monitor which combines a novel aerosol concentrator, a micro-particle collector, and a microfluidic sample processing and detection platform customized to perform a broad range of assays, coupled to automated data recording and/or transmitting equipment and a position-monitoring device (such as a GPS unit) to provide time- and space-resolved measurements of a wide range of environmental agents. This exposure monitor will significantly improve the ability to localize exposure events in space and time and will provide a critical tool for use in resolving uncertainties about the relationships between exposure and human health impacts. In the proposed project we will customize our aerodynamic lens aerosol concentrator technology for use in a portable air sampler, we will integrate the aerosol concentrator with a capillary aerosol particle collector, and we will interface this particle collection system to a microfluidics card where biological or chemical assays will be carried out and the results detected. Enertechnix, Inc. will be responsible for adapting its existing aerosol concentration and collection technology and Micronics, Inc. will adapt its commercial microfluidics card technology for use in a portable exposure monitor. The University of Washington will develop specific assays that can be adapted for implementation in the microfluidics environment for detection of target biological and chemical agents. Aerosol handling components will be developed by Enertechnix using engineering and CFD modeling capabilities previously developed for other Government-funded projects. These components will be tested using an existing, fully instrumented aerosol chamber and a variety of standard diagnostic methods for particle and bacterial detection such as epi-fluorescence microscopy, bulk fluorescence, culture, real-time PCR, etc. Microfluidic circuitry will be designed and prototyped by Micronics using methods and tools previously developed for production of similar microfluidic cards employing nucleic acid and immunological assays and electrochemical and other detection methods. Specific assays will be developed by the University of Washington using its extensive expertise and laboratory facilities in the areas of microbiology, chemistry, environmental science (atmospheric pollutant detection and characterization), etc.

描述（由申请人提供） 拟议项目将开发和演示一种个人暴露监测器，该监测器结合了一种新型气溶胶浓缩器、一种微粒收集器以及一种定制的微流体样品处理和检测平台，可进行广泛的分析，连接到自动数据记录和/或传输设备以及位置监视装置（如全球定位系统装置）提供对各种环境因素的时间和空间分辨测量。这一接触监测器将大大提高在空间和时间上确定接触事件地点的能力，并将提供一个关键工具，用于解决接触与人类健康影响之间关系的不确定性。 在拟议的项目中，我们将定制我们的空气动力学透镜气溶胶浓缩器技术用于便携式空气采样器，我们将集成气溶胶浓缩器与毛细管气溶胶颗粒收集器，我们将这个颗粒收集系统接口到微流控卡，在那里将进行生物或化学分析和检测结果。Enertechnix公司将负责调整其现有的气溶胶浓缩和收集技术，将其商用微流控卡技术用于便携式曝光监测器。华盛顿大学将开发特定的检测方法，可以适用于在微流体环境中检测目标生物和化学制剂。 气溶胶处理部件将由Enertechnix利用以前为其他政府资助项目开发的工程和计算流体动力学建模能力开发。这些组件将使用现有的全仪器化气溶胶室和各种用于颗粒和细菌检测的标准诊断方法进行测试，如落射荧光显微镜、批量荧光、培养、实时PCR、微流控电路将由微流控技术使用先前开发的用于生产类似微流控卡的方法和工具来设计和原型化，所述微流控卡采用核酸和免疫学测定，电化学和其它检测方法。华盛顿大学将利用其在微生物学、化学、环境科学（大气污染物检测和定性）等领域的广泛专门知识和实验室设施，开发具体的化验方法。