A Rapid Label-free Sensor for Immune Markers of Environmental Exposure

一种快速无标记传感器，用于检测环境暴露的免疫标记物

• 批准号: 7676433
• 负责人: Christopher Striemer
• 金额: $ 17.02万
• 依托单位: PATHOLOGICS, LLC
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-24 至 2009-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7676433
• 关键词: Acute respiratory infection; Address; Affect; Airborne Particulate Matter; Albumins; Amines; Animal Model; Animals; Antibodies; Benchmarking; Binding; Binding Sites; Biological; Biological Assay; Biosensing Techniques; Blood; Blood specimen; Bovine Serum Albumin; Buffers; Cardiovascular Diseases; Chemicals; Chemistry; Chronic; Computers; Cosmetics; Data; Detection; Development; Devices; Disease; Elements; Environmental Exposure; Enzyme-Linked Immunosorbent Assay; Esters; Event; Exposure to; Fingers; Fire - disasters; Funding Opportunities; Gastrointestinal tract structure; Glutaral; Goals; Growth; Health; Hour; Human; Human Activities; Image; Immune system; Immunologic Markers; Individual; Interferons; Label; Literature; MYO5A gene; Malignant neoplasm of lung; Measurement; Measures; Methods; Molecular; Monoclonal Antibodies; Mucous body substance; Nanotechnology; Operative Surgical Procedures; Paint; Particulate Matter; Pathologic; Performance; Phase; Physiological; Process; Protein Microchips; Proteins; Protocols documentation; Range; Reader; Relative (related person); Research; Research Design; Risk; Saliva; Sampling; Sensitivity and Specificity; Serum; Signaling Protein; Silicon Dioxide; Site; Skin; Solutions; Source; Specificity; Staging; Standards of Weights and Measures; Structure; Sunscreening Agents; Surface; Techniques; Technology; Testing; Time; Toxic effect; Triton X100; Tweens; Ultrafine; United States Environmental Protection Agency; Universities; Urine; Validation; antigen antibody binding; base; biochip; commercial application; concept; consumer product; cross reactivity; density; design; environmental agent; experience; forest; improved; instrument; laptop; prototype; respiratory; response; sensor; size; success; surfactant; urban area

DESCRIPTION (provided by applicant) Airborne particulate matter (PM) derived from a variety of natural sources (volcanic activity, forest fires) is omnipresent. However, human activity since the industrial revolution and the advent of the internal combustion engine has substantially increased the concentration of PM, particularly in urban areas. Human derived PM tends to be of smaller size, facilitating entry into the body through the respiratory and digestive tracts, as well as through the skin. It also tends to have adsorbed chemical species with greater toxicity than its natural counterparts. The explosive growth of nanotechnology in research and consumer products (sunscreens, cosmetics, paint) is also creating an array of ultrafine materials with largely unknown health effects. Despite numerous studies implicating PM in various disorders including acute respiratory infections, lung cancer, and chronic respiratory and cardiovascular diseases, the specific biological mechanisms involved are not fully understood. Pathologics seeks to leverage its proprietary Arrayed Imaging Reflectometery (AIR) biosensing platform to build a device capable of capturing the detailed physiological response to PM exposure by profiling key marker proteins in conveniently available biosamples such as a finger pick of blood, urine, mucous, saliva, etc. Currently, such testing takes hours and is often done with individual tests. AIR is capable of sensitive and simultaneous protein detection with a device that is currently the size of a laptop computer. The low complexity of the testing platform will also enable the development of handheld units that would be particularly useful for profiling exposure response in the field. In addition to saving time and offering greater convenience these multiplex sensors will allow far more molecular data to be collected than is currently feasible.

描述（由申请人提供） 来自各种自然来源（火山活动，森林火灾）的空气中颗粒物（PM）无处不在。然而，自工业革命以来的人类活动和内燃机的出现大大增加了PM的浓度，特别是在城市地区。人体来源的PM往往尺寸较小，便于通过呼吸道和消化道以及通过皮肤进入体内。它还往往具有吸附的化学物种，其毒性比天然对应物更大。纳米技术在研究和消费品（防晒霜，化妆品，油漆）中的爆炸性增长也创造了一系列超细材料，这些材料对健康的影响基本上是未知的。尽管许多研究表明PM与各种疾病有关，包括急性呼吸道感染、肺癌、慢性呼吸道和心血管疾病，但所涉及的具体生物学机制尚未完全了解。Pathologics试图利用其专有的阵列成像反射仪（AIR）生物传感平台来构建一种能够通过分析方便获得的生物样本（如血液，尿液，粘液，唾液等）中的关键标记蛋白来捕获对PM暴露的详细生理反应的设备。AIR能够用目前笔记本电脑大小的设备进行灵敏和同步的蛋白质检测。测试平台的低复杂性还将使手持装置的开发成为可能，这对于在现场分析暴露反应特别有用。除了节省时间和提供更大的便利性，这些多重传感器将允许收集比目前可行的更多的分子数据。