A rapid label-free sensor for immune markers of environmental exposure for applic

描述(申请人提供)：来自各种自然来源(火山活动、森林火灾)的空气颗粒物(PM)无处不在。然而，自工业革命和内燃机问世以来，人类活动大大增加了PM的浓度，特别是在城市地区。人源性PM往往体积较小，便于通过呼吸道和消化道以及通过皮肤进入人体。它也往往具有比天然化合物更大的毒性的吸附化学物种。纳米技术在研究和消费产品(防晒霜、化妆品、油漆)领域的爆炸性增长，也创造了一系列对健康有很大影响的超细材料。尽管大量研究表明PM与多种疾病有关，包括急性呼吸道感染、肺癌以及慢性呼吸系统和心血管疾病，但其具体的生物学机制尚不完全清楚。Adarza BioSystems，Inc.寻求利用其专有的阵列成像反射计量(AIR)生物传感平台来构建一种设备，通过分析方便获得的生物样本中的关键标记蛋白(可能是&gt；100)来快速捕获PM暴露的详细生理响应。目前，此类测试需要数小时，通常通过单独的单链测试完成。AIR能够用目前笔记本电脑大小的设备同时灵敏地检测蛋白质。这项提议的目标是将我们的多重实验室化验结果密封在一个保护性的塑料试剂盒中，以便在不可预测和没有保护的环境中进行实地工作将变得现实。我们打算与一家在制造符合军事规范的便携式生物检测方面有着长期记录的公司合作，以促进这一开发工作。还将设计具有集成处理单元的改进的光学读取器，以控制盒内的流体操作。最后，将使用新开发的空气生物测试和更标准的实验室测试来验证空气生物测试系统的性能和性能，并对先前人口研究中使用的1800个样本进行测试。到这个为期两年的项目结束时，我们打算有一个现场可部署的原型和分析试剂盒，为大规模人口研究做好准备。 公共卫生相关性：颗粒物(PM)是环境医学领域的一个主要问题，因为通过空气、水或接触接触到小颗粒是不可避免的，而且这些物质已知通过呼吸道和消化道进入人体，甚至通过皮肤进入人体。PM暴露与各种呼吸、炎症和心脏疾病有关，但其潜在机制尚不清楚。Adarza生物系统公司寻求将其快速和高度多元化的蛋白质图谱技术集成到一个现场可部署的系统中，该系统能够在大规模人群研究中测量参与者对颗粒物或其他环境压力的生理反应。

DESCRIPTION (provided by applicant): Airborne particulate matter (PM) derived from a variety of natural sources (volcanic activity, forest fires) is omnipresent. However, human activity since the industrial revolution and the advent of the internal combustion engine has substantially increased the concentration of PM, particularly in urban areas. Human derived PM tends to be of smaller size, facilitating entry into the body through the respiratory and digestive tracts, as well as through the skin. It also tends to have adsorbed chemical species with greater toxicity than its natural counterparts. The explosive growth of nanotechnology in research and consumer products (sunscreens, cosmetics, paint) is also creating an array of ultrafine materials with largely unknown health effects. Despite numerous studies implicating PM in various disorders including acute respiratory infections, lung cancer, and chronic respiratory and cardiovascular diseases, the specific biological mechanisms involved are not fully understood. Adarza BioSystems, Inc. seeks to leverage its proprietary Arrayed Imaging Reflectometery (AIR) biosensing platform to build a device capable of rapidly capturing the detailed physiological response to PM exposure by profiling key marker proteins (potentially >100) in conveniently available biosamples such as a finger pick of blood, urine, mucous, saliva, etc. Currently, such testing takes hours and is often done with individual singleplex tests. AIR is capable of sensitive and simultaneous protein detection with a device that is currently the size of a laptop computer. The goal of this proposal is to take our multiplexed laboratory assay, and seal it in a protective plastic cartridge so that fieldwork in unpredictable and unprotected environments would become realistic. We intend to collaborate with a company that has a long track record in building portable bioassays to military specifications to facilitate this development effort. An improved optical reader will also be designed with an integrated processing unit to control fluidic operations within the cartridge. Finally, a bank of ~1800 samples used in the previous population study will be tested using both the newly developed AIR bioassay and a more standard laboratory assay to verify the performance and of the AIR bioassay system. By the end of this 2-year project we intend to have a field deployable prototype and assay cartridges that are ready for large population studies. PUBLIC HEALTH RELEVANCE: Particulate matter (PM) is a major concern in the field of environmental medicine because exposure to small particles through air, water, or contact is unavoidable and these materials are known to enter the body through the respiratory and digestive tract, and even through skin. Exposure to PM has been linked to a variety of respiratory, inflammatory, and cardiac disorders, but the underlying mechanisms are not well understood. Adarza Biosystems, Inc. seeks to integrate its rapid and highly multiplexed protein profiling technology, in a field- deployable system capable of measuring a participant's physiological response to particulate matter or other environmental stressors in large population studies.