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ENZYMATIC LUMINESCENCE microRNA ASSAY

酶促发光 microRNA 测定

基本信息

批准号：
7413598
负责人：
YE SUN
金额：
$ 10万
依托单位：
SCI-TEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2010-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7413598
关键词：
Address Adopted Area Arts Biochemical Reaction Biological Biological Assay Biological Process Biological Sciences Bioluminescence Cell physiology Collaborations Complementary DNA Computer software DNA DNA Sequence Determination DNA copy number Data Set Detection Development Diabetes Mellitus Diphosphates Disease Equipment Family Genomics Goals Immune response Investigation Laboratories Lead Length Letters Light Link Luciferases Malignant Neoplasms Manufacturer Name Measures Messenger RNA Methodology MicroRNAs Molecular Analysis Monitor Nucleotides Numbers One-Step dentin bonding system Pattern Performance Phase Photons Polymerase Polymerase Chain Reaction Protocols documentation RNA Range Reaction Reagent Reporting Research Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Role playing therapy Sampling Series Signal Transduction Site Small Business Funding Mechanisms Small Business Innovation Research Grant Small RNA Sulfate Adenylyltransferase System Techniques Technology Tennessee Testing Time Tube United States National Institutes of Health Universities Visible Radiation Work base clinical Diagnosis commercialization concept cost cost effective design drug discovery high throughput analysis innovation instrument instrumentation luciferin luminescence nucleic acid detection programs research facility research study response

项目摘要

DESCRIPTION (provided by applicant): The goal of this project is to develop a new, highly sensitive, and cost-effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expands pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay has unique sensitivity and dynamic range and is expected to outperform real-time PCR in applications for analysis of small RNA molecules. The qELA requires less expensive reagents and equipment than RT-PCR and microarrays and can be used in a number of commercial assays for application in life sciences research, drug discovery, and clinical diagnosis.

描述（由申请人提供）：该项目的目标是开发一种新的，高灵敏度和成本效益的RNA酶促发光检测（qELA），用于生物样品中microRNA的高通量检测和定量。该测定实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，用于高灵敏度和准确定量小RNA分子。该方法具有独特的灵敏度和动态范围，有望在小RNA分子分析应用中优于实时PCR。qELA需要比RT-PCR和微阵列更便宜的试剂和设备，并且可以用于许多商业测定，用于生命科学研究，药物发现和临床诊断。