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ENZYMATIC LUMINESCENCE microRNA ASSAY

酶促发光 microRNA 测定

基本信息

批准号：
8062092
负责人：
YE SUN
金额：
$ 38.52万
依托单位：
SCI-TEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2013-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8062092
关键词：
Area Biochemical Reaction Biological Biological Assay Biological Models Biological Sciences Bioluminescence Buffers Carbon Dioxide Clinical Collaborations Complementary DNA DNA Detection Development Diphosphates Equipment Genomics Goals Individual Laboratories Laboratory Research Letters Light Luciferases Manufacturer Name Marketing Methodology Methods MicroRNAs Mission Monitor National Institute of Biomedical Imaging and Bioengineering National Institute of Drug Abuse National Institute of General Medical Sciences Nucleic Acids Nucleotides Pathway interactions Performance Phase Photons Polymerase Polymerase Chain Reaction Progress Reports RNA Reaction Reagent Research Sampling Series Small RNA Sulfate Adenylyltransferase System Techniques Technology Temperature Tennessee Time United States National Institutes of Health Universities Validation Visible Radiation Work anticancer research base clinical Diagnosis clinical application cost effective drug discovery high throughput screening instrumentation luciferin luminescence new technology nucleic acid detection polymerization protocol development public health relevance technology development

项目摘要

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay can match the performance of the real time quantitative PCR technique (rt-qPCR) in many applications and is simpler and more cost effective. The technology can be implemented in a number of commercial assays for application in life science research, drug discovery, and clinical diagnosis. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a new high performance cost effective bioluminescence assay for microRNA analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis by 1,800 clinical laboratories and thousands of life science research laboratories in US.

描述（由申请人提供）：该项目的目标是开发一种新的高灵敏度和高成本效益的RNA酶促发光法（qELA），用于高通量检测和定量生物样品中的microRNA。该分析实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，用于高灵敏度和准确的小RNA分子定量。所提出的分析方法在许多应用中可以与实时定量PCR技术（rt-qPCR）的性能相匹配，并且更简单，更具成本效益。该技术可用于生命科学研究、药物发现和临床诊断等多种商业分析。