ENZYMATIC LUMINESCENCE microRNA ASSAY

描述（由申请人提供）：该项目的目标是开发一种新的高灵敏度且具有成本效益的 RNA 酶促发光测定法 (qELA)，用于生物样品中 microRNA 的高通量检测和定量。该测定采用与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，可实现小 RNA 分子的高灵敏度和准确定量。所提出的检测方法在许多应用中可以与实时定量 PCR 技术 (rt-qPCR) 的性能相匹配，并且更简单、更具成本效益。该技术可用于生命科学研究、药物发现和临床诊断等领域的多种商业化验。 公共健康相关性：该项目的目标是开发一种用于 microRNA 分析的新型高性能、经济有效的生物发光测定方法。所提出的检测方法为目前在市场上占主导地位的实时聚合酶链反应技术提供了技术先进且成本显着降低的替代方案。所提出的生物发光测定法具有通用性，可用于美国 1,800 个临床实验室和数千个生命科学研究实验室的生命科学研究、药物发现、临床诊断等各种应用。

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay can match the performance of the real time quantitative PCR technique (rt-qPCR) in many applications and is simpler and more cost effective. The technology can be implemented in a number of commercial assays for application in life science research, drug discovery, and clinical diagnosis. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a new high performance cost effective bioluminescence assay for microRNA analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis by 1,800 clinical laboratories and thousands of life science research laboratories in US.