ENZYMATIC LUMINESCENCE microRNA ASSAY

描述(申请人提供)：本项目的目标是开发一种新的高灵敏度和低成本的RNA酶发光分析方法(QELA)，用于高通量检测和定量生物样品中的microRNA。该检测实现了与焦化测序相同的检测概念，但扩展了焦化测序检测方法，以实现对小RNA分子的高灵敏度和准确定量。所提出的方法在许多应用中可以与实时定量聚合酶链式反应(RT-qPCR)相媲美，并且更简单、更经济。这项技术可以应用于生命科学研究、药物发现和临床诊断的许多商业分析中。 公共卫生相关性：该项目的目标是开发一种新的高性能、高性价比的用于microRNA分析的生物发光分析方法。建议的检测方法为目前在市场上占主导地位的实时聚合酶链式反应技术提供了技术先进、成本低得多的替代方案。所提出的生物发光分析方法具有普适性，可用于美国1800个临床实验室和数千个生命科学研究实验室的生命科学研究、药物研发、临床诊断等领域。

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay can match the performance of the real time quantitative PCR technique (rt-qPCR) in many applications and is simpler and more cost effective. The technology can be implemented in a number of commercial assays for application in life science research, drug discovery, and clinical diagnosis. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a new high performance cost effective bioluminescence assay for microRNA analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis by 1,800 clinical laboratories and thousands of life science research laboratories in US.