ENZYMATIC LUMINESCENCE microRNA ASSAY

描述（由申请人提供）：该项目的目标是开发一种新的高灵敏度和高成本效益的RNA酶促发光法（qELA），用于高通量检测和定量生物样品中的microRNA。该分析实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，用于高灵敏度和准确的小RNA分子定量。所提出的分析方法在许多应用中可以与实时定量PCR技术（rt-qPCR）的性能相匹配，并且更简单，更具成本效益。该技术可用于生命科学研究、药物发现和临床诊断等多种商业分析。

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay can match the performance of the real time quantitative PCR technique (rt-qPCR) in many applications and is simpler and more cost effective. The technology can be implemented in a number of commercial assays for application in life science research, drug discovery, and clinical diagnosis. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a new high performance cost effective bioluminescence assay for microRNA analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis by 1,800 clinical laboratories and thousands of life science research laboratories in US.