ENZYMATIC LUMINESCENCE microRNA ASSAY

描述（由申请人提供）：本项目的目标是开发一种新的高灵敏度和成本效益的RNA酶促发光测定（qELA），用于生物样品中microRNA的高通量检测和定量。该检测试剂盒实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，用于高灵敏度和准确定量小RNA分子。所提出的测定法在许多应用中可以与真实的时间定量PCR技术（rt-qPCR）的性能相匹配，并且更简单且更具成本效益。该技术可以在许多商业测定中实施，以应用于生命科学研究、药物发现和临床诊断。 公共卫生相关性：该项目的目标是开发一种新的高性能、高性价比的生物发光检测方法，用于microRNA分析。所提出的测定法为真实的时间聚合酶链反应技术提供了技术先进且显著更便宜的替代方案，该技术目前在市场上占主导地位。所提出的生物发光测定是通用的，并且可以被美国1，800个临床实验室和数千个生命科学研究实验室用于生命科学研究、药物发现、临床诊断中的各种应用。

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay can match the performance of the real time quantitative PCR technique (rt-qPCR) in many applications and is simpler and more cost effective. The technology can be implemented in a number of commercial assays for application in life science research, drug discovery, and clinical diagnosis. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a new high performance cost effective bioluminescence assay for microRNA analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis by 1,800 clinical laboratories and thousands of life science research laboratories in US.