DNA MICROARRAY AND EXPRESSION CORE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 DNA微阵列和表达核心为已经进行实验或重新进行此类实验的研究人员提供生物信息学专业知识。该核心去年升级了其功能和基础设施，并配备了GenePix 4000 B双激光微阵列扫描仪（扫描分辨率为5 nm），由GenePix Pro 6.0图像软件和Acuity数据库支持，在专用的戴尔PC上运行。该核心还可以访问用于功能基因组学的Spotfire DecisionSite和用于数据挖掘的R-code/Bioconductor，并将收购Incidity Pathways Analysis软件。核心具有专用的网络驱动器访问权限，用于数据存储、检索和分发。 该核心目前为研究界提供以下服务：a）微阵列实验设计B）斑点微阵列实验c）PCR阵列d）数据分析e）RNA扩增f）蛋白质表达和纯化。此外，该中心希望在未来扩展到以下研究服务领域：a）基因沉默; B）定制微阵列c）蛋白质组学。 DNA微阵列和表达核心正在积极与TNPRC的其他组成部分合作。我们与病毒诊断核心合作开发了一种基于微阵列的检测试剂，用于在TNPRC的恒河猴菌落中定植的微生物病原体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Microarray and Expression core provides bioinformatics expertise to investigators who have already performed their experiments, or performs such experiments de novo. The core upgraded its capabilities and infrastructure last year, and is equipped with a GenePix 4000B dual-laser microarray scanner (scan resolution of 5 nm), supported by GenePix Pro 6.0 image software and Acuity database, operating on a dedicated Dell PC. The core also has access to Spotfire DecisionSite for Functional Genomics and R-code/Bioconductor for data-mining, and will be acquiring Ingenuity Pathways Analysis software. The core has dedicated access to a network drive for data storage, retrieval and distribution. The core currently provides the following services to the research community: a) Microarray Experimental Design b) Spotted Microarray Experiments c) PCR arrays d) Data Analysis e) RNA Amplification: f) Protein Expression and Purification. In addition the core would like to expand into the following research service areas in future: a) Gene silencing; b) Custom microarray c) Proteomics. The DNA Microarray and expression core is actively collaborating with other components of TNPRC. We have developed a proposal in collaboration with the Viral Diagnostics core to generate a microarray based detection reagent for microbial pathogens that colonize the rhesus colony at TNPRC.