SURVEY OF ENZOOTIC PATHOGENS AND ARTHROPOD VECTORS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项调查旨在评估TNPRC室外繁殖群体中已知的地方性病原体的流行水平，该项目作为群体健康的哨兵。从每只动物身上采集的血液和粪便样本被检查是否有感兴趣的寄生虫。粪便样本通过直接涂片法和浓缩法进行检查。血液样本通过厚薄血液涂片检查疟原虫种类和克诺特试验检查丝虫病。用酶联免疫吸附试验检测血浆中是否有克氏锥虫的证据，并保存DNA以供将来研究。潜在的媒介每周被CDC灯光陷阱和妊娠陷阱捕获。蚊子的分类和检查通过试纸ELISA法或新开发的西尼罗河病毒(WNV)LAMP试验进行。2006年，我们检查了442份粪便样本和590份血液样本。我们还保存并检测了208份克鲁兹旋毛虫的血清样本，发现了34只动物，显示出接触克鲁兹旋毛虫的证据。我们已经能够在体外培养一些受感染动物的寄生虫，目前正在对受感染动物的母亲重新取样，以寻找这种寄生虫垂直传播的证据。这些动物中的许多都是在该中心出生的，这表明传播是通过当地的三叶虫传播的。今年我们花了几个月的时间尝试在夜间诱捕病媒，但到目前为止还没有成功。其他感兴趣的病原体包括flleborni类圆线虫、蓝氏贾第鞭毛虫、结肠巴兰蒂虫、鞭虫和老鼠绦虫。我们已经扩大了抽样范围，通过展示S.F.lleborni的垂直传播，将婴儿母亲的乳汁包括在内。这种寄生虫已被证明存在于非洲妇女的乳汁中。它在猿猴妈妈的乳汁中的存在将表明需要开发新的治疗方法，以帮助减少这种微生物在我们繁殖群体中造成的发病率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A survey designed to assess the level of endemicity of known enzootic pathogens in the outdoor breeding colonies at the TNPRC, this project serves as a sentinel for the health of the colony. Samples of blood and stool obtained from each animal are examined for parasites of interest. Stool samples are examined by a direct smear and a concentration method. Blood samples are examined by thick and thin blood smears for Plasmodium species and the Knott's test for filariasis. Plasma is examined by ELISA for evidence of Trypanosoma cruzi and DNA is saved for future studies. Potential vectors are trapped weekly by CDC light traps and gravid traps. Mosquitoes are speciated and examined by dipstick ELISA test or the newly-developed LAMP test for West Nile virus (WNV). During 2006 we examined 442 stool samples and 590 blood samples. We have also saved and tested 208 serum samples for T. cruzi and have found 34 animals which show evidence of exposure to T. cruzi. We have been able to culture parasites in vitro from some infected animals and are presently re-sampling mothers of infected animals to look for evidence of vertical transmission of this parasite. Many of these animals were born at the Center indicating transmission is taking place here with local triatomid bugs. We spent some months this year attempting the trap the vector at night but have so far been unsuccessful. Other pathogens of interest include Strongyloides f¿lleborni, Giardia lamblia, Balantidium coli, Trichuris trichiura, and Hymenolepis diminuta, the rat tapeworm. We have expanded our sampling to include milk from mothers with babies by to demonstrate vertical transmission of S. f¿lleborni. This parasite has been shown to be present in the milk of African women. Its presence in the milk of simian mothers will be an indication that new treatment approaches need to be developed to help reduce the morbidity caused by this organism in our breeding colony.