SURVEY OF ENZOOTIC PATHOGENS AND ARTHROPOD VECTORS

地方性病原体和节肢动物媒介的调查

• 批准号: 7562284
• 负责人: FRANK B. COGSWELL
• 金额: $ 3.04万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562284
• 关键词: African; Animals; Anticoagulants; Arthropod Vectors; Balantidium; Blood specimen; Breeding; Centers for Disease Control and Prevention (U.S.); Computer Retrieval of Information on Scientific Projects Database; Culicidae; DNA; Enzyme-Linked Immunosorbent Assay; Exposure to; Feces; Filariasis; Funding; Future; Giardia lamblia; Grant; Health; In Vitro; Institution; Light; Methods; Milk; Morbidity - disease rate; Mothers; Organism; Parasites; Patient currently pregnant; Plasma; Plasmodium; Rat tapeworm; Research; Research Personnel; Resources; Sampling; Sentinel; Serum; Source; Strongyloides; Surveys; Testing; Thick; Trichocephalus trichiura; Trypanosoma cruzi; United States National Institutes of Health; Vertical Disease Transmission; West Nile virus; Woman; design; enzootic; interest; pathogen; transmission process; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A survey designed to assess the level of endemicity of known enzootic pathogens in the outdoor breeding colonies at the TNPRC, this project serves as a sentinel for the health of the colony. Samples of blood and stool obtained from each animal are examined for parasites of interest. Stool samples are examined by a direct smear and a concentration method. Blood samples are examined by thick and thin blood smears for Plasmodium species and the Knott's test for filariasis. Plasma is examined by ELISA for evidence of Trypanosoma cruzi and DNA is saved for future studies. Potential vectors are trapped weekly by CDC light traps and gravid traps. Mosquitoes are speciated and examined by dipstick ELISA test or the newly-developed LAMP test for West Nile virus (WNV). During 2006 we examined 442 stool samples and 590 blood samples. We have also saved and tested 208 serum samples for T. cruzi and have found 34 animals which show evidence of exposure to T. cruzi. We have been able to culture parasites in vitro from some infected animals and are presently re-sampling mothers of infected animals to look for evidence of vertical transmission of this parasite. Many of these animals were born at the Center indicating transmission is taking place here with local triatomid bugs. We spent some months this year attempting the trap the vector at night but have so far been unsuccessful. Other pathogens of interest include Strongyloides f¿lleborni, Giardia lamblia, Balantidium coli, Trichuris trichiura, and Hymenolepis diminuta, the rat tapeworm. We have expanded our sampling to include milk from mothers with babies by to demonstrate vertical transmission of S. f¿lleborni. This parasite has been shown to be present in the milk of African women. Its presence in the milk of simian mothers will be an indication that new treatment approaches need to be developed to help reduce the morbidity caused by this organism in our breeding colony.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这一调查旨在评估TNPRC室外养殖场已知地方病病原体的地方性水平，该项目是殖民地健康状况的监测点。检查从每只动物获得的血液和粪便样品中的感兴趣的寄生虫。粪便样本通过直接涂片和浓缩法进行检查。通过厚血涂片和薄血涂片检查血液样本中的疟原虫，并通过诺特氏试验检查丝虫病。通过ELISA检查血浆中克氏锥虫的证据，并保存DNA用于将来的研究。潜在的病媒每周用CDC灯诱捕器和妊娠诱捕器诱捕。蚊子的物种和检查试纸ELISA测试或新开发的LAMP测试西尼罗河病毒（WNV）。2006年，我们检查了442份粪便样本和590份血液样本。我们还保存并检测了208份血清样本的T。cruzi和已经发现了34只动物显示出暴露于T。克鲁兹我们已经能够在体外培养一些受感染动物的寄生虫，目前正在重新采样受感染动物的母亲，以寻找这种寄生虫垂直传播的证据。这些动物中有许多是在该中心出生的，这表明传播是在这里与当地的锥虫发生的。今年我们花了几个月的时间试图在夜间捕捉矢量，但迄今为止没有成功。其他感兴趣的病原体包括类圆线虫、贾第鞭毛虫、结肠小袋纤毛虫、鞭毛虫和鼠绦虫。 我们已经扩大了我们的采样范围，包括母乳与婴儿，以证明垂直传播的S。我的天。这种寄生虫已被证明存在于非洲妇女的乳汁中。它存在于猿猴母亲的乳汁中，这表明需要开发新的治疗方法来帮助减少这种生物体在我们的繁殖群体中引起的发病率。