SURVEY OF ENZOOTIC PATHOGENS AND ARTHROPOD VECTORS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该项目是一项调查，旨在评估TNPRC室外养殖群体中已知的地方性病原体的流行水平。从每只动物身上采集的血液和粪便样本被检查是否有感兴趣的寄生虫。粪便样本通过直接涂片法和浓缩法进行检查。血液样本通过厚薄血液涂片检查疟原虫种类和克诺特试验检查丝虫病。用酶联免疫吸附试验检测血浆中是否有克氏锥虫的证据，并保存DNA以供将来研究。潜在媒介每周被CDC灯光陷阱和妊娠陷阱捕获。蚊子的分类和检查通过试纸ELISA法或新开发的西尼罗河病毒(WNV)LAMP试验进行。在2005年期间，我们检查了838份粪便样本和733份血液样本。我们还保存并检测了766个克鲁兹旋毛虫的血清样本，发现了34只动物有接触克鲁兹旋毛虫的证据。我们已经能够在体外培养一些受感染动物的寄生虫，目前正在对受感染动物的母亲重新取样，以寻找这种寄生虫垂直传播的证据。这些动物中的许多都是在该中心出生的，这表明传播是通过当地的三叶虫传播的，尽管媒介尚未确定。其他发现的感兴趣的病原体包括：圆线虫、蓝氏贾第鞭毛虫、结肠巴兰氏菌、鞭虫和小膜虫。我们最近扩大了抽样范围，以包括有婴儿的母亲的母乳，我们打算通过这些母乳来证明S.f.lleborni的垂直传播。同样的寄生虫已被证明存在于非洲妇女的乳汁中。它在猿猴妈妈的乳汁中的存在将表明，需要开发新的治疗方法，以帮助减少这种微生物在我们繁殖群体中造成的发病率，特别是在幼年动物中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is a survey designed to assess the level of endemicity of known enzootic pathogens in the outdoor breeding colonies at the TNPRC. Samples of blood and stool obtained from each animal are examined for parasites of interest. Stool samples are examined by a direct smear and a concentration method. Blood samples are examined by thick and thin blood smears for Plasmodium species and the Knott's test for filariasis. Plasma is examined by ELISA for evidence of Trypanosoma cruzi and DNA is saved for future studies. Potential vectors are trapped weekly by CDC light trap and gravid traps. Mosquitoes are speciated and examined by dipstick ELISA test or the newly-developed LAMP test for West Nile virus (WNV). During 2005 we examined 838 stool samples and 733 blood samples. We have also saved and tested 766 serum samples for T. cruzi and have found 34 animals which show evidence of exposure to T. cruzi. We have been able to culture parasites in vitro from some infected animals and are presently re-sampling mothers of infected animals to look for evidence of vertical transmission of this parasite. Many of these animals were born at the Center indicating transmission is taking place here with local triatomid bugs, although the vector has not yet been identified. Other pathogens of interest found include Strongyloides f¿lleborni, Giardia lamblia, Balantidium coli, Trichuris trichiura, and Hymenolepis diminuta, the rat tapeworm. We have recently expanded our sampling to include milk from mothers with babies by which we intend to demonstrate vertical transmission of S. f¿lleborni. This same parasite has been shown to be present in the milk of African women. Its presence in the milk of simian mothers will be an indication that new treatment approaches need to be developed to help reduce the morbidity caused by this organism in our breeding colony, especially in young animals.