IDENTIFICATION OF HEPATITIS B VIRUS SUBGENOTYPE A3 IN RURAL GABON

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在中非加蓬赤道森林的五个偏远村庄进行了B型肝炎病毒（HBV）分子调查。311名居民中有270人（86.8%）是HBV感染者或有既往HBV感染的证据。在27例（8.6%）HBV感染受试者中疑似慢性肝炎（对应于B表面抗原（HBsAg）阳性）。高HBV病毒载量主要见于4岁儿童7年对13例患者进行了前S/S区序列测定，12例为HBV-A基因型。在一个病例中，我们发现了基因型A和E之间重组的证据。系统发育分析表明，加蓬的HBV毒株不同于HBV-A亚型（A1和A2）。这些来自加蓬的新HBV毒株与先前报道的来自喀麦隆和刚果民主共和国的HBV-A3亚型毒株聚类。前S2结构域的分析使我们能够确定中非HBV-A3亚型毒株特有的两个氨基酸取代（N/152/S和N/174/T）和这些新的加蓬HBV-A3亚型分离株特有的一个氨基酸取代（P/155/Q）。两个新的加蓬HBV分离株的两个全基因组序列也被提出，并确认了独特的HBV-Gab-A3簇。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A hepatitis B virus (HBV) molecular survey was conducted in five remote villages in the equatorial forest in Gabon, Central Africa. Two hundred seventy out of 311 inhabitants (86.8%) were HBV-infected or had evidence of past HBV infection. Chronic hepatitis corresponding to hepatitis B surface antigen (HBsAg) positivity was suspected in 27 (8.6%) of the HBV-infected subjects. High HBV viral loads were detected mainly in children aged 47 years. The pre-S/S domains were sequenced in 13 cases and 12 strains belonged to HBV-A genotype. In one case we found evidence for recombination between genotypes A and E. Phylogenetic analysis revealed that Gabonese HBV strains were distinct from HBV-A subgenotypes (A1 and A2). These new HBV strains from Gabon clustered with previously reported HBV-A3 subgenotype strains from Cameroon and Democratic Republic of Congo. The analysis of the pre-S2 domain allowed us to determine two amino acid substitutions (N/152/S and N/174/T) specific to the Central African HBV-A3 subgenotype strains and one amino acid substitution (P/155/Q) unique to these new Gabonese HBV-A3 subgenotype isolates. Two full genome sequences of two new Gabonese HBV isolates are also presented and confirm the distinctive HBV-Gab-A3 cluster.