FIBRONECTIN-INTEGRIN BINDING & POST MENSTRUAL REPAIR IN THE MACAQUE ENDOMETRIUM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的目标是更好地了解正常月经和月经失调，包括月经过多和突破性出血。月经是子宫内膜破坏和再生的周期性过程，由月经周期结束时黄体酮(P)的急性退出而开始。一些研究表明，停磷会引发炎症样反应，包括上调白细胞介素8(IL-8)和单核细胞趋化蛋白-1(MCP-1)，从而刺激月经期间中性粒细胞、单核细胞和巨噬细胞的募集。在月经后期和月经周期的子宫内膜修复阶段，细胞外基质蛋白纤维连接蛋白在子宫内膜最高功能区的表达也显著上调。我们推测，纤维连接蛋白-整合素结合可能在调节炎症反应中发挥作用，并在停止月经出血过程中进一步发挥重要作用。本研究有两个特定的目的：目的1研究纤维连接蛋白、整合素和炎性趋化因子受体在恒河猴月经期间子宫内膜中的表达模式。目的2确定纤维连接蛋白-整合素阻断对月经和子宫内膜修复相关基因调控的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to better understand normal menstruation and menstrual disorders including menorrhagia and breakthrough bleeding. Menstruation is a cyclical process of endometrial destruction and regeneration, initiated by an acute withdrawal of progesterone (P) at the end of the menstrual cycle. Several studies indicate that P withdrawal triggers an inflammation-like response that includes up-regulation of interleukin-8 (IL 8) and monocyte chemotactic protein-1 (MCP 1) that can stimulate the recruitment of neutrophils, monocytes and macrophages during menses. The extracellular matrix protein fibronectin is also dramatically up-regulated in the uppermost functionalis zones of the endometrium during late menstruation and the endometrial repair phase of the menstrual cycle. We hypothesize that fibronectin-integrin binding may play a role in regulating the inflammatory response and further have essential actions during the cessation of menstrual bleeding. This study has two specific aims: Aim 1 is to characterize the pattern of fibronectin, integrin and inflammatory chemokine receptor expression in the macaque endometrium during menses. Aim 2 is to determine the effect of fibronectin-integrin blockade on the regulation of genes associated with menstruation and endometrial repair.