ROLE OF THE ANTIZYME FAMILY DURING XENOPUS DEVELOPMENT

抗酶家族在非洲爪蟾发育过程中的作用

• 批准号: 7725143
• 负责人: Charles Richard Toth
• 金额: $ 3.11万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725143
• 关键词: Anabolism; Binding; Catabolism; Cell Proliferation; Cells; Charge; Complement; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Enzymes; Family; Funding; Genes; Genetic Screening; Goals; Grant; Institution; Nature; Organism; Ornithine Decarboxylase; Pathway interactions; Play; Polyamines; Process; Proteins; Rate; Reaction; Regulation; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Saccharomycetales; Source; Testing; Toxic effect; United States National Institutes of Health; Xenopus; Yeasts; ornithine decarboxylase antizyme; uptake; yeast genetics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polyamines are charged organic molecules that are vitally important for macromolecular reactions in cells but are toxic at high levels. There exist well-regulated biosynthetic pathways for both polyamine anabolism and catabolism. A protein involved in the control of polyamine levels, antizyme (AZ), plays a role in regulating polyamine levels in cells. AZ lowers polyamines below toxic levels by inhibiting polyamine uptake and activity of the rate-limiting enzyme ornithine decarboxylase (ODC) via direct binding and targeted degradation of ODC. By understanding the function of AZ in a normal setting, one can better understand the role of AZ in a disease setting and determine the nature of its toxicity in controlling cell proliferation A tractable organism for polyamine research is the budding yeast Saccharomyces cerevisiae. The regulation of polyamine synthesis in yeast has been delineated although not specifically the role of antizyme in the process. In addition, mammalian forms of the polyamine genes can complement their functional equivalents in yeast cells. Using a yeast genetic screen, genes that functionally interact with AZ and its regulation of ODC can be isolated and characterized. This study proposes to use a genetic screen to obtain yeast genes that play a role in the regulation and function of the ODC/AZ pathway in yeast. The goal of the project is: (1) determine the functional role that AZ plays in proliferation in Saccharomyces cerevisiae by a genetic screen. The goal will be used to test the following hypothesis: AZ regulates the proliferative capacity of cells by controlling ODC levels.

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