KANSAS U COBRE TRANSGENIC & GENOTYPING CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 转基因和基因靶向设施为COBRE研究人员提供许多研究支持服务，因此被添加为核心设施。 服务包括通过原核注射产生转基因小鼠，胚胎干细胞中的基因靶向，通过胚泡显微注射产生嵌合小鼠和通过聚合酶链反应进行基因分型。 由于执行这些服务所需的设备和专业知识都很昂贵，因此建立一个核心设施使所有COBRE调查人员都能够获得这些技术和随后的研究模型。 转基因设施还有助于为COBRE研究人员设计实验，并通过COBRE的资助扩大了向研究人员提供的服务范围。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Transgenic and Gene-Targeting Facility provides many research support services to COBRE investigators and was therefore added as a Core Facility. Services include the generation of transgenic mice by pronuclear injection, gene targeting in embryonic stem cells, generation of chimeric mice by blastocyst microinjection and genotyping by Polymerase Chain Reaction. Because both the equipment and expertise required to perform these services is costly, establishing a Core Facility enables all COBRE investigators access to these techniques and subsequent research models. The Transgenic Facility has also been instrumental in designing experiments for COBRE investigators, and has expanded the range of services offered to investigators via funding from the COBRE.