KANSAS U COBRE: TRANSGENIC & GENOTYPING CORE

堪萨斯大学 COBRE：转基因

• 批准号: 7959570
• 负责人: Melissa Larson
• 金额: $ 14.82万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959570
• 关键词: Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Cryopreservation; Development; Electroporation; Equipment; Funding; Gene Targeting; Gene-Modified; Generations; Genotype; Grant; Individual; Injection of therapeutic agent; Institution; Kansas; Laboratories; Molecular; Mus; Operative Surgical Procedures; Regulation; Research; Research Personnel; Research Support; Resources; Services; Source; Techniques; Time; Training; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; blastocyst; cost; embryonic stem cell; gene function; in vivo; member; skills; sperm cell; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The gene-modified mouse has become a standard and indispensable tool for investigating the function of genes in vivo. Although the modified mouse is common, the techniques employed for its generation require skill and specialized equipment. It is far more cost- and time-effective to centralize both the equipment and skill into a core, shared, research support facility, as has been done here, rather than equipping and training individual laboratories. By centralizing operations, the gene-modified mouse is available as a research tool to all investigators. The Transgenic and Gene-Targeting Facility provides services to COBRE investigators that include generation of transgenic mice by pronuclear injection, generation of chimeric mice by blastocyst injection, targeting of embryonic stem cells by electroporation, cryopreservation of sperm from gene-modified mice and genotyping of gene-modified mouse litters. All of these services have been requested by and performed for members of the COBRE.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 转基因小鼠已经成为研究基因在体内功能的标准和不可或缺的工具。尽管改良的老鼠很常见，但它的产生所采用的技术需要技能和专门的设备。像这里所做的那样，将设备和技能集中到一个核心的、共享的研究支持设施中，比装备和培训单独的实验室更具成本效益和时间效益。通过集中操作，所有研究人员都可以使用转基因小鼠作为研究工具。转基因和基因打靶设施为Cobre研究人员提供服务，包括通过原核注射产生转基因小鼠、通过囊胚注射产生嵌合体小鼠、通过电穿孔瞄准胚胎干细胞、冷冻保存转基因小鼠的精子以及对转基因小鼠后代进行基因分型。所有这些服务都是由科布雷成员要求并为其提供的。