Transgenic and Gener-Targeting Core

核心B：转基因和基因靶向核心 转基因和基因打靶核心集成并增强了我们现有的转基因和基因打靶设备的能力，在创造转基因和基因敲除小鼠方面取得了公认的成功记录。这一核心将通过为遗传小鼠模型的产生、表征和维护提供具有成本效益的服务来协助Cobre研究人员。在该中心的32名高级科学家中，有9名经常使用核心，5个Cobre初级调查员项目中的2个也有 计划利用这一设施。这一核心将为科布雷科学家提供将DNA显微注射到受精卵中并将胚胎转移到受精卵中以创造转基因小鼠的服务。此外，该中心还将提供创造嵌合体小鼠的服务，包括维持几个胚胎干细胞系、胚胎干细胞的电穿孔和选择、核型测定和选定胚胎干细胞的囊胚注射。科布雷 资金将用于扩大这些服务，包括操纵和制备用于显微注射或基因靶向的DNA，以及转基因和基因敲除小鼠的基因分型。Core还提供小鼠品系的冷冻保存。Cobre基金将为初级调查人员和中心成员提供服务补贴，KUMC将继续为这一设施提供重要的机构支持。该设施的工作人员包括一名技术主任、科学主任和两名研究助理，他们将负责 与两名Cobre联合顾问

Core B: Transgenic and Gene Targeting Core The Transgenic and Gene Targeting Core integrates with and enhances the capabilities of our exisitng Transgenic and Gene Targeting Facility with a proven track record of success in creating transgenic and knockout mice. This Core will assist COBRE investigators by providing cost-efffective serviecs for the generation, characterization, and maintenance of genetic mouse models. Ofthe 32 senior scientists in the Center, 9 are frequent users ofthe Core, and 2 of the 5 COBRE Beginning Investigator projects also have plans to utilize this facility. This Core will provide COBRE scientists with the service to microinject DNA into fertilized eggs and transfer embryos to recipient females to create transgenic mice. In addition, this Core will provide services to create chimeric mice, including the maintenance of several ES cell lines, electroporation and selection of ES cells, karyotype determination and blastocyst injection of selected ES cells. COBRE funds will be used to expand these services to include the manipulation and preparation of DNA for microinjection or gene targeting, and genotyping of transgenic and knockout mice. The Core also provides cryopreservation of mouse lines. The COBRE funds will subsidize services for Beginning Investigators and Center members and KUMC will continue to provide significant institutional support for this facility. The Facility is staffed by a Technical Director, Scientific Director, and two Research Associates, who will interface with two COBRE co-advisors