权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Transgenic and Gener-Targeting Core

转基因和基因靶向核心

基本信息

批准号：
9100875
负责人：
Melissa Larson
金额：
$ 16.2万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2018-06-30
项目状态：
已结题

项目摘要

Core B: Transgenic and Gene Targeting Core The Transgenic and Gene Targeting Core integrates with and enhances the capabilities of our exisitng Transgenic and Gene Targeting Facility with a proven track record of success in creating transgenic and knockout mice. This Core will assist COBRE investigators by providing cost-efffective serviecs for the generation, characterization, and maintenance of genetic mouse models. Ofthe 32 senior scientists in the Center, 9 are frequent users ofthe Core, and 2 of the 5 COBRE Beginning Investigator projects also have plans to utilize this facility. This Core will provide COBRE scientists with the service to microinject DNA into fertilized eggs and transfer embryos to recipient females to create transgenic mice. In addition, this Core will provide services to create chimeric mice, including the maintenance of several ES cell lines, electroporation and selection of ES cells, karyotype determination and blastocyst injection of selected ES cells. COBRE funds will be used to expand these services to include the manipulation and preparation of DNA for microinjection or gene targeting, and genotyping of transgenic and knockout mice. The Core also provides cryopreservation of mouse lines. The COBRE funds will subsidize services for Beginning Investigators and Center members and KUMC will continue to provide significant institutional support for this facility. The Facility is staffed by a Technical Director, Scientific Director, and two Research Associates, who will interface with two COBRE co-advisors.

核心B：转基因和基因靶向核心转基因和基因靶向核心整合并增强了我们的转基因和基因靶向设施的能力，并在创造转基因和基因敲除小鼠方面取得了成功。该核心将通过为遗传小鼠模型的生成、表征和维护提供具有成本效益的服务来协助COBRE研究人员。在该中心的32名高级科学家中，有9名是Core的经常用户，5个COBRE Beginning Investigator项目中的2个也有计划利用这一设施。该核心将为COBRE科学家提供将DNA显微注射到受精卵中并将胚胎转移到受体雌性中以创建转基因小鼠的服务。此外，该核心将提供创建嵌合小鼠的服务，包括几种ES细胞系的维护，ES细胞的电穿孔和选择，核型确定和所选ES细胞的囊胚注射。Cobre 资金将用于扩大这些服务，包括用于显微注射或基因靶向的DNA操作和制备，以及转基因和基因敲除小鼠的基因分型。核心还提供小鼠品系的冷冻保存。COBRE基金将资助初级研究人员和中心成员的服务，KUMC将继续为该设施提供重要的机构支持。该设施的工作人员包括一名技术主任、科学主任和两名研究助理，他们将与COBRE的两名共同顾问进行交流。