WATER CHANNEL AQUAPORIN-3 IN BM-DERIVED EPIDERMAL CELLS PLAY ROLE WOUND HEALING

BM 来源的表皮细胞中的水通道 Aquaporin-3 在伤口愈合中发挥作用

• 批准号: 7725257
• 负责人: YINSHENG WAN
• 金额: $ 1.51万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-05 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725257
• 关键词: Animals; Basal Cell; Cell Differentiation process; Cell Lineage; Cell division; Cells; Cellular biology; Computer Retrieval of Information on Scientific Projects Database; Cutaneous; Epidermis; Epithelium; Exhibits; Family; Fibroblasts; Funding; Glycerol; Grant; Hair follicle structure; Homeostasis; Human; Institution; Integral Membrane Protein; Mammals; Morphogenesis; Movement; Multipotent Stem Cells; Numbers; Pathology; Pathway interactions; Physiology; Plants; Play; Population; Property; Research; Research Personnel; Resources; Role; Role playing therapy; Sebaceous Glands; Skin; Skin Cancer; Skin Physiology; Source; Stem cells; TGF Beta Signaling Pathway; Testing; Therapeutic; United States National Institutes of Health; Water; Wound Healing; aquaporin 3; c-myc Genes; cell motility; college; keratinocyte; member; notch protein; professor; reconstitution; self-renewal; stem cell fate; water channel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Wan is presently an assistant professor at Providence College, which is only 2 miles from our institution. Water channel aquaporins (AQPs) are a family of small, hydrophobic, integral membrane proteins that are expressed widely in animal and plant kingdoms, with 13 members having thus far been identified in mammals. Aquaporin-3 (AQP3), functionally expressing in human skin keratinocytes and fibroblasts, facilitates water and glycerol movement into and out of the skin and plays an important role in skin physiology and pathology. However, the possible role of AQPs in human epidermal stem cells has not been fully explored. Stem cells are characterized by their prolonged self-renewal capacity and by their asymmetric replication. Asymmetric replication is a special property of stem cells: with every cell division, one of the cells retains its self-renewing capacity, whereas the other enters a differentiation pathway and joins a mature non-dividing population. Epidermis of the skin contains a subpopulation of basal cells called epidermal stem cells that exhibit the properties expected of somatic stem cells. Epidermal stem cells are multipotent in that they can reconstitute all the components of the cutaneous epithelium: epidermis, hair follicles and sebaceous glands. They are easily accessible and hold great promise for therapeutic applications in skin homeostasis and wound repair as well as skin cancer. A large number of factors have been implicated in skin morphogenesis and the determination of epidermal stem cell fate. Besides the Wnt/Lef/Tcf pathway and c-myc, two play critical roles in epidermal stem cell lineage determination and proliferation, they include p63, hairless and members of the Shh, Notch and TGF-beta-signalling pathways. However the possible role of AQPs in epidermal stem cell biology or physiology is not fully studied. The specific aims are: 1. To test the expression of AQP1, AQP3 or AQP4 in human epidermal stem cells; 2. To test the possible involvement of AQPs in human epidermal stem cells migration; 3. To test the possible role of AQPs in human epidermal stem cell renewal or proliferation potential; 4. To test the role of AQPs in epidermal stem cells differentiation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 博士Wan目前是普罗维登斯学院的助理教授，该学院距离我们的机构仅2英里。 水通道水通道蛋白（Water channel aquaporins，AQP）是一类广泛存在于动物和植物中的小分子疏水性膜蛋白，目前已在哺乳动物中鉴定出13个成员。水通道蛋白3（Aquaporin-3，AQP 3）在人皮肤角质形成细胞和成纤维细胞中功能性表达，促进水和甘油进出皮肤，在皮肤生理和病理中起重要作用。然而，水通道蛋白在人类表皮干细胞中的可能作用尚未得到充分探讨。 干细胞的特征在于其延长的自我更新能力和其不对称复制。不对称复制是干细胞的一种特殊性质：随着每次细胞分裂，其中一个细胞保持其自我更新能力，而另一个细胞进入分化途径并加入成熟的非分裂群体。皮肤的表皮包含称为表皮干细胞的基底细胞亚群，其表现出体干细胞的预期特性。 表皮干细胞是多能的，因为它们可以重建皮肤上皮的所有组成部分：表皮、毛囊和皮脂腺。它们很容易获得，并且在皮肤稳态和伤口修复以及皮肤癌的治疗应用中具有很大的前景。大量的因素已经牵连在皮肤形态发生和表皮干细胞命运的决定。除了Wnt/Lef/Tcf通路和c-myc外，还有两个在表皮干细胞谱系确定和增殖中起关键作用，它们包括p63、无毛和Shh、Notch和TGF-β信号通路的成员。然而，AQPs在表皮干细胞生物学或生理学中的可能作用尚未得到充分研究。 具体目标是：1.检测AQP 1、AQP 3、AQP 4在人表皮干细胞中的表达。探讨水通道蛋白在人表皮干细胞迁移中的作用。检测水通道蛋白在人表皮干细胞更新或增殖潜能中的可能作用。探讨水通道蛋白在表皮干细胞分化中的作用。