DESIGN AND ELABORATION OF A PHOTO-ACTIVATABLE DE NOVO PROTEIN

点击翻译按钮获取中文摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of a collaboration between Professors Michael J. Therien, Jeffrey G. Saven, and William F. DeGrado, we generate four-helix protein bundles capable of binding photoactivateable inorganic complexes that possess zinc diphenyl porphyrin, (DPP)Zn moieties. These protein/chromophore systems undergo photoinitiated electron/energy transfer processes and exhibit excellent non-linear optic properties. As part of the characterization of the photodynamics, we utilize the Nd:YAG laser system in the Regional Laser and Biomedical Technologies Laboratories (RLBL) for measuring the triplet excited state processes of the various protein/chromophore complexes.