DESIGN AND ELABORATION OF A PHOTO-ACTIVATABLE DE NOVO PROTEIN

光激活从头蛋白的设计和制备

• 批准号: 7723847
• 负责人: MICHAEL J THERIEN
• 金额: $ 0.52万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723847
• 关键词: Binding; Biomedical Technology; Collaborations; Complex; Computer Retrieval of Information on Scientific Projects Database; Electrons; Energy Transfer; Exhibits; Funding; Grant; Helix (Snails); Institution; Laboratories; Lasers; Measures; Optics; Porphyrins; Process; Property; Proteins; Research; Research Personnel; Resources; Source; System; Triplet Multiple Birth; United States National Institutes of Health; Zinc; chromophore; design; diphenyl; ear helix; professor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of a collaboration between Professors Michael J. Therien, Jeffrey G. Saven, and William F. DeGrado, we generate four-helix protein bundles capable of binding photoactivateable inorganic complexes that possess zinc diphenyl porphyrin, (DPP)Zn moieties. These protein/chromophore systems undergo photoinitiated electron/energy transfer processes and exhibit excellent non-linear optic properties. As part of the characterization of the photodynamics, we utilize the Nd:YAG laser system in the Regional Laser and Biomedical Technologies Laboratories (RLBL) for measuring the triplet excited state processes of the various protein/chromophore complexes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 作为 Michael J. Therien、Jeffrey G. Saven 和 William F. DeGrado 教授之间合作的一部分，我们生成了能够结合具有二苯基锌卟啉 (DPP)Zn 部分的可光活化无机复合物的四螺旋蛋白束。 这些蛋白质/发色团系统经历光引发电子/能量转移过程，并表现出优异的非线性光学特性。 作为光动力学表征的一部分，我们利用区域激光和生物医学技术实验室 (RLBL) 的 Nd:YAG 激光系统来测量各种蛋白质/发色团复合物的三重激发态过程。