USING NATURALLY OCCURRING PROTEINS AS STABLE SCAFFOLDS FOR STUDYING PROTEIN FOLD

使用天然存在的蛋白质作为研究蛋白质折叠的稳定支架

• 批准号: 7721791
• 负责人: BRIAN W MATTHEWS
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721791
• 关键词: Address; Amyloid; Computer Retrieval of Information on Scientific Projects Database; Dependence; Elements; Funding; Grant; Institution; Muramidase; Mutation; Protein Engineering; Proteins; Research; Research Personnel; Resources; Side; Source; United States National Institutes of Health; Vertebral column; design; protein folding; response; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal contains three projects involving genetically engineered proteins designed to address questions about protein folding and stability. The first project addresses the question of how the sidechains of amyloid forming sequences interact by solublizing these sequences via their incorporation in T4 lysozyme. The second project addresses questions about of the context dependence of secondary structural elements. The last project addresses the plasticity of protein interiors by studying the magnitude and direction of shifts in backbone and side chain atoms that occur in response to mutations suggested by automated design in the case of the T4 lysozyme.

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该提案包含三个涉及基因工程蛋白质的项目，旨在解决蛋白质折叠和稳定性问题。 第一个项目解决了淀粉样蛋白形成序列的侧链如何通过将这些序列掺入T4溶菌酶中而使其溶解而相互作用的问题。 第二个项目解决了二级结构元素的上下文依赖性问题。 最后一个项目通过研究T4溶菌酶自动设计建议的突变引起的骨架和侧链原子变化的幅度和方向来解决蛋白质内部的可塑性。