XAS CHARACTERIZATION OF THE DETERMINANTS OF METAL-SITE STRUCTURE IN PROTEINS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 XAS将用于表征与从头设计的肽结合的各种重金属的结构。在大多数情况下，XAS是提供金属位点直接结构信息的唯一方法。这项工作的直接目标是确定金属位点结构的不同决定因素的相对重要性-即，金属特异性立体化学偏好与肽强化的结构偏好。这项工作的最终目标是开发一个详细的了解，参与控制金属蛋白金属特异性的因素，特别是因为它涉及到金属离子稳态和重金属毒性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. XAS will be used to characterize the structure of a variety of heavy metals bound to de novo designed peptides. In most cases, XAS is the only method available to provide direct structural information for the metal-site. The immediate goal of this work is to define the relative importance of the different determinants of metal-site structure - i.e., metal-specific stereochemical preference vs. peptide-enforced structural preferences. The ultimate goal of this work is to develop a detailed understanding of the factors involved in controlling metalloprotein metal-specificity, particularly as it relates to metal-ion homeostsis and heavy-metal toxicity.