CHARACTERIZATION OF THE ROLE OF METAL IONS IN RNA FOLDING AND ACTIVATION

金属离子在 RNA 折叠和激活中的作用表征

• 批准号: 8362179
• 负责人: James E. Penner-Hahn
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362179
• 关键词: Adopted; Complex; DNA; Divalent Cations; Funding; Grant; Ions; Label; Metal Binding Site; Metals; National Center for Research Resources; Nature; Play; Principal Investigator; RNA; RNA Folding; Radiation; Research; Research Infrastructure; Resources; Role; Source; Structure; Testing; United States National Institutes of Health; Vertebral column; cost; inorganic phosphate; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Divalent cations play a critical role in the folding and activation of RNA. Unlike DNA, where the canonical structure is well defined, RNAs are found to adopt a wide range of structures. In many cases, divalent cations (Mg2+, Mn2+, and Zn2+) are required for the RNA to adopt a biologically active structure. Recent NMR studies have provided direct evidence for a unique binding site of the metal to the RNA and have suggested that the metal forms an inner-sphere complex with the RNA. EXAFS will be used to test this proposal and to better define the nature of the RNA-metal interaction. In order to define specifically the RNA-metal binding site, thio-modified RNA will be used to label possible backbone phosphates.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 二价阳离子在RNA的折叠和激活中起着关键作用。与DNA不同，RNA的典型结构被很好地定义，RNA被发现采用广泛的结构。在许多情况下，二价阳离子（Mg 2+、Mn 2+和Zn 2+）是RNA采用生物活性结构所必需的。最近的核磁共振研究提供了直接的证据，一个独特的结合位点的金属的RNA，并建议，金属形成一个内球复合物的RNA。EXAFS将用于测试这一提议，并更好地定义RNA-金属相互作用的性质。为了特异性地定义RNA-金属结合位点，硫代修饰的RNA将用于标记可能的骨架磷酸盐。